LOCUS CR456893 825 bp mRNA linear HUM 21-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0111D for
gene MAD2L1BP, MAD2L1 binding protein; complete cds, incl.
stopcodon.
ACCESSION CR456893
VERSION CR456893.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 825)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 825)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0111D, ORFNo 1225
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0111D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_014628 we found amino acid
exchange(s) at position (first base of changed triplet):
820(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..825
/db_xref="H-InvDB:HIT000267743"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0111D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..825
/codon_start=1
/gene="MAD2L1BP"
/db_xref="GOA:Q15013"
/db_xref="H-InvDB:HIT000267743.11"
/db_xref="HGNC:HGNC:21059"
/db_xref="InterPro:IPR009511"
/db_xref="PDB:2QYF"
/db_xref="PDB:6F0X"
/db_xref="UniProtKB/Swiss-Prot:Q15013"
/protein_id="CAG33174.1"
/translation="MAAPEAEVLSSAAVPDLEWYEKSEETHASQIELLETSSTQEPLN
ASEAFCPRDCMVPVVFPGPVSQEGCCQFTCELLKHIMYQRQQLPLPYEQLKHFYRKPS
PQAEEMLKKKPRATTEVSSRKCQQALAELESVLSHLEDFFARTLVPRVLILLGGNALS
PKEFYELDLSLLAPYSVDQSLSTAACLRRLFRAIFMADAFSELQAPPLMGTVVMAQGH
RNCGEDWFRPKLNYRVPSRGHKLTVTLSCGRPSIRTTAWEDYIWFQAPVTFKGFRD"
BASE COUNT 192 a 237 c 216 g 180 t
ORIGIN
1 atggcggcgc cggaggcgga ggttctgtcc tcagccgcag tccctgattt ggagtggtat
61 gagaagtccg aagaaactca cgcctcccag atagaactac ttgagacaag ctctacgcag
121 gaacctctca acgcttcgga ggccttttgc ccaagagact gcatggtacc agtggtgttt
181 cctgggcctg tgagccagga aggctgctgt cagtttactt gtgaacttct aaagcatatc
241 atgtatcaac gccagcagct ccctctgccc tatgaacagc ttaagcactt ttaccgaaaa
301 ccttctcccc aggcagagga gatgctgaag aagaaacctc gggccaccac tgaggtgagc
361 agcaggaaat gccaacaagc cctggcagaa ctggagagtg tcctcagcca cctggaggac
421 ttctttgcac ggacactagt accgcgagtg ctgattctcc ttgggggcaa tgccctaagc
481 cccaaggagt tctatgaact cgacttgtct ctgctggccc cctacagcgt ggaccagagc
541 ctgagcacag cagcttgttt gcgccgtctc ttccgagcca tattcatggc tgatgccttt
601 agcgagcttc aggctcctcc actcatgggc accgtcgtca tggcacaggg acaccgcaac
661 tgtggagaag attggtttcg acccaagctc aactatcgag tgcccagccg gggccataaa
721 ctgactgtga ccctgtcatg tggcagacct tccatccgaa ccacggcttg ggaagactac
781 atttggttcc aggcaccagt gacatttaaa ggcttccgcg attaa
//