LOCUS CR456882 552 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0815D for
gene SMAP, small acidic protein; complete cds, incl. stopcodon.
ACCESSION CR456882
VERSION CR456882.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 552)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 552)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0815D, ORFNo 1203
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0815D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_014267 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..552
/db_xref="H-InvDB:HIT000267732"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0815D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..552
/codon_start=1
/gene="SMAP"
/db_xref="GOA:O00193"
/db_xref="H-InvDB:HIT000267732.12"
/db_xref="HGNC:HGNC:16990"
/db_xref="InterPro:IPR026714"
/db_xref="InterPro:IPR028124"
/db_xref="UniProtKB/Swiss-Prot:O00193"
/protein_id="CAG33163.1"
/translation="MSAARESHPHGVKRSASPDDDLGSSNWEAADLGNEERKQKFLRL
MGAGKKEHTGRLVIGDHKSTSHFRTGEEDKKINEELESQYQQSMDSKLSGRYRRHCGL
GFSEVEDHDGEGDVAGDDDDDDDDSPDPESPDDSESDSESEKEESAEELQAAEHPDEV
EDPKNKKDAKSNYKMMFVKSSGS"
BASE COUNT 192 a 93 c 147 g 120 t
ORIGIN
1 atgagtgctg ccagagagtc tcacccgcat ggggtgaagc gttcagcctc cccagacgac
61 gatctgggat ctagcaattg ggaggcagca gacttgggta atgaagagag aaaacaaaag
121 ttcttgagac ttatgggtgc aggaaagaaa gaacatactg gtcgtcttgt tataggagat
181 cacaaatcaa catctcactt ccgaaccggg gaagaagaca agaaaattaa tgaagaactg
241 gagtctcaat atcagcaaag tatggacagt aaattatcag gaagatatcg gcgacattgt
301 ggacttggct tcagtgaggt agaagaccat gatggagaag gtgatgtggc tggagatgat
361 gatgatgacg atgatgattc acctgatcct gaaagtccag atgattctga aagcgattca
421 gagtcagaga aagaagaatc tgctgaagaa ctccaagctg ctgagcaccc tgatgaagtg
481 gaggatccca aaaacaaaaa agatgcaaaa agcaattata aaatgatgtt tgttaaatcc
541 agtggttctt aa
//