LOCUS CR456873 387 bp mRNA linear HUM 03-JUN-2004 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F116D for gene RPL22, ribosomal protein L22; complete cds, incl. stopcodon. ACCESSION CR456873 VERSION CR456873.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 387) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 387) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834F116D, ORFNo 1192 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F116D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_000983 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..387 /db_xref="H-InvDB:HIT000267723" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834F116D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..387 /codon_start=1 /gene="RPL22" /db_xref="GOA:P35268" /db_xref="H-InvDB:HIT000267723.14" /db_xref="HGNC:HGNC:10315" /db_xref="InterPro:IPR002671" /db_xref="InterPro:IPR038526" /db_xref="PDB:4UG0" /db_xref="PDB:4V6X" /db_xref="PDB:5AJ0" /db_xref="PDB:5LKS" /db_xref="PDB:5T2C" /db_xref="PDB:6EK0" /db_xref="PDB:6IP5" /db_xref="PDB:6IP6" /db_xref="PDB:6IP8" /db_xref="PDB:6OLE" /db_xref="PDB:6OLF" /db_xref="PDB:6OLG" /db_xref="PDB:6OLI" /db_xref="PDB:6OLZ" /db_xref="PDB:6OM0" /db_xref="PDB:6OM7" /db_xref="PDB:6QZP" /db_xref="UniProtKB/Swiss-Prot:P35268" /protein_id="CAG33154.1" /translation="MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQ ERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRV VANSKESYELRYFQINQDEEEEEDED" BASE COUNT 132 a 65 c 105 g 85 t ORIGIN 1 atggctcctg tgaaaaagct tgtggtgaag gggggcaaaa aaaagaagca agttctgaag 61 ttcactcttg attgcaccca ccctgtagaa gatggaatca tggatgctgc caattttgag 121 cagtttttgc aagaaaggat caaagtgaac ggaaaagctg ggaaccttgg tggaggggtg 181 gtgaccatcg aaaggagcaa gagcaagatc accgtgacat ccgaggtgcc tttctccaaa 241 aggtatttga aatatctcac caaaaaatat ttgaagaaga ataatctacg tgactggttg 301 cgcgtagttg ctaacagcaa agagagttac gaattacgtt acttccagat taaccaggac 361 gaagaagagg aggaagacga ggattaa //