LOCUS CR456859 423 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G106D for
gene ZNF547, zinc finger protein 547; complete cds, incl.
stopcodon.
ACCESSION CR456859
VERSION CR456859.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 423)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 423)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G106D, ORFNo 1145
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G106D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC008889 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..423
/db_xref="H-InvDB:HIT000267709"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G106D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..423
/codon_start=1
/gene="ZNF547"
/db_xref="GOA:Q6IBE5"
/db_xref="H-InvDB:HIT000267709.14"
/db_xref="InterPro:IPR006722"
/db_xref="InterPro:IPR011012"
/db_xref="UniProtKB/TrEMBL:Q6IBE5"
/protein_id="CAG33140.1"
/translation="MSGSFYFVIVGHHDNPVFEMEFLPAGKAESKDDHRHLNQFIAHA
ALDLVDENMWLSNNMYLKTVDKFNEWFVSAFVTAGHMRFIMLHDIRQEDGIKNFFTDV
YDLYIKFSMNPFYEPNSPIRSSAFDRKVQFLGKKHLLS"
BASE COUNT 127 a 75 c 87 g 134 t
ORIGIN
1 atgtctggaa gcttctactt tgtaattgtt ggtcaccatg ataatccagt ttttgaaatg
61 gagtttttgc cagctgggaa ggcagaatcc aaagacgacc atcgtcatct gaaccagttc
121 atagctcatg ctgctctcga cctcgtagat gagaacatgt ggctgtcgaa caacatgtac
181 ttgaaaactg tggacaagtt caacgagtgg tttgtgtcag catttgtcac cgcggggcat
241 atgaggttta ttatgcttca tgacataaga caagaagatg gaataaagaa cttctttact
301 gatgtttatg atttatatat aaaattttca atgaatccat tttatgaacc caattctcct
361 attcgatcaa gtgcatttga cagaaaagtt cagtttcttg ggaagaaaca ccttttaagt
421 taa
//