LOCUS CR456850 1527 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C1111D for
gene HMGCS2, 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2
(mitochondrial); complete cds, incl. stopcodon.
ACCESSION CR456850
VERSION CR456850.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1527)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1527)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C1111D, ORFNo 1119
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C1111D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC044217 we found amino acid
exchange(s) at position (first base of changed triplet):
700(his->tyr) 1153(ser->thr)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1527
/db_xref="H-InvDB:HIT000267700"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C1111D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1527
/codon_start=1
/gene="HMGCS2"
/db_xref="GOA:P54868"
/db_xref="H-InvDB:HIT000267700.13"
/db_xref="HGNC:HGNC:5008"
/db_xref="InterPro:IPR000590"
/db_xref="InterPro:IPR010122"
/db_xref="InterPro:IPR013528"
/db_xref="InterPro:IPR013746"
/db_xref="InterPro:IPR016039"
/db_xref="PDB:2WYA"
/db_xref="UniProtKB/Swiss-Prot:P54868"
/protein_id="CAG33131.1"
/translation="MQRLLTPVKRILQLTRAVQETSLTPARLLPVAHQRFSTASAVPL
AKTDTWPKDVGILALEVYFPAQYVDQTDLEKYNNVEAGKYTVGLGQTRMGFCSVQEDI
NSLCLTVVQRLMERIQLPWDSVGRLEVGTETIIDKSKAVKTVLMELFQDSGNTDIEGI
DTTNACYGGTASLFNAANWMESSSWDGRYAMVVCGDIAVYPSGNARPTGGAGAVAMLI
GPKAPLALERGLRGTYMENVYDFYKPNLASEYPIVDGKLSIQCYLRALDRCYTSYRKK
IQNQWKQAGSDRPFTLDDLQYMIFHTPFCKMVQKSLARLMFNDFLSASSDTQTSLYKG
LEAFGGLKLEDTYTNKDLDKALLKASQDMFDKKTKASLYLSTHNGNMYTSTLYGCLAS
LLSHHSAQELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLA
SRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV
"
BASE COUNT 372 a 421 c 385 g 349 t
ORIGIN
1 atgcagcgtc tgttgactcc agtgaagcgc attctgcaac tgacaagagc ggtgcaggaa
61 acctccctca cacctgctcg cctgctccca gtagcccacc aaaggttttc tacagcctct
121 gctgtccccc tggccaaaac agatacttgg ccaaaggacg tgggcatcct ggccctggag
181 gtctacttcc cagcccaata tgtggaccaa actgacctgg agaagtataa caatgtggaa
241 gcaggaaagt atacagtggg cttgggccag acccgtatgg gcttctgctc agtccaagag
301 gacatcaact ccctgtgcct gacggtggtg caacggctga tggagcgcat acagctccca
361 tgggactctg tgggcaggct ggaagtaggc actgagacca tcattgacaa gtccaaagct
421 gtcaaaacag tgctcatgga actcttccag gattcaggca atactgatat tgagggcata
481 gataccacca atgcctgcta cggtggtact gcctccctct tcaatgctgc caactggatg
541 gagtccagtt cctgggatgg tcgttatgcc atggtggtct gtggagacat tgccgtctat
601 cccagtggta atgctcgtcc cacaggtggg gccggagctg tggctatgct gattgggccc
661 aaggcccctc tggccctgga gcgagggctg aggggaacct atatggagaa tgtgtatgac
721 ttctacaaac caaatttggc ctcggagtac ccaatagtgg atgggaagct ttccatccag
781 tgctacttgc gggccttgga tcgatgttac acatcatacc gtaaaaaaat ccagaatcag
841 tggaagcaag ctggcagcga tcgacccttc acccttgacg atttacagta catgatcttt
901 catacaccct tttgcaagat ggtccagaag tctctggctc gcctgatgtt caatgacttc
961 ctgtcagcca gcagtgacac acaaaccagc ttatataagg ggctggaggc tttcgggggg
1021 ctaaagctgg aagacaccta caccaacaag gacctggata aagcacttct aaaggcctct
1081 caggacatgt tcgacaagaa aaccaaggct tccctttacc tctccactca caatgggaac
1141 atgtacacct caaccctgta cgggtgcctg gcctcgcttc tgtcccacca ctctgcccaa
1201 gaactggctg gctccaggat tggtgccttc tcttatggct ctggtttagc agcaagtttc
1261 ttttcatttc gagtatccca ggatgctgct ccaggctctc ccctggacaa gttggtgtcc
1321 agcacatcag acctgccaaa acgcctagcc tcccgaaagt gtgtgtctcc tgaggagttc
1381 acagaaataa tgaaccaaag agagcaattc taccataagg tgaatttctc cccacctggt
1441 gacacaaaca gccttttccc aggtacttgg tacctggagc gagtggacga gcagcatcgc
1501 cgaaagtatg cccggcgtcc cgtttaa
//