LOCUS CR456825 678 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0315D for
gene EEF1B2, eukaryotic translation elongation factor 1 beta 2;
complete cds, incl. stopcodon.
ACCESSION CR456825
VERSION CR456825.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 678)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 678)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0315D, ORFNo 1053
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0315D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_021121 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..678
/db_xref="H-InvDB:HIT000267675"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0315D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..678
/codon_start=1
/gene="EEF1B2"
/db_xref="GOA:P24534"
/db_xref="H-InvDB:HIT000267675.12"
/db_xref="HGNC:HGNC:3208"
/db_xref="InterPro:IPR001326"
/db_xref="InterPro:IPR014038"
/db_xref="InterPro:IPR014717"
/db_xref="InterPro:IPR018940"
/db_xref="InterPro:IPR036219"
/db_xref="InterPro:IPR036282"
/db_xref="PDB:1B64"
/db_xref="PDB:5DQS"
/db_xref="UniProtKB/Swiss-Prot:P24534"
/protein_id="CAG33106.1"
/translation="MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSP
PPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDID
LFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEE
CVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMD
VAAFNKI"
BASE COUNT 209 a 130 c 185 g 154 t
ORIGIN
1 atgggtttcg gagacctgaa aagccctgcc ggcctccagg tgctcaacga ttacctggcg
61 gacaagagct acatcgaggg gtatgtgcca tcacaagcag atgtggcagt atttgaagcc
121 gtgtccagcc caccgcctgc cgacttgtgt catgccctac gttggtataa tcacatcaag
181 tcttacgaaa aggaaaaggc cagcctgcca ggagtgaaga aagctttggg caaatatggt
241 cctgccgatg tggaagacac tacaggaagt ggagctacag atagtaaaga tgatgatgac
301 attgacctct ttggatctga tgatgaggag gaaagtgaag aagcaaagag gctaagggaa
361 gaacgtcttg cacaatatga atcaaagaaa gccaaaaaac ctgcacttgt tgccaagtct
421 tccatcttac tagatgtgaa accttgggat gatgagacag atatggcgaa attagaggag
481 tgcgtcagaa gcattcaagc agacggctta gtctggggct catctaaact agttccagtg
541 ggatacggaa ttaagaaact tcaaatacag tgtgtagttg aagatgataa agttggaaca
601 gatatgctgg aggagcagat cactgctttt gaggactatg tgcagtccat ggatgtggct
661 gctttcaaca agatttaa
//