LOCUS CR456812 1164 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0118D for
gene BBOX1, butyrobetaine (gamma), 2-oxoglutarate dioxygenase
(gamma-butyrobetaine hydroxylase) 1; complete cds, incl. stopcodon.
ACCESSION CR456812
VERSION CR456812.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1164)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1164)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H0118D, ORFNo 1014
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0118D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_003986 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1164
/db_xref="H-InvDB:HIT000267662"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H0118D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1164
/codon_start=1
/gene="BBOX1"
/db_xref="GOA:O75936"
/db_xref="H-InvDB:HIT000267662.11"
/db_xref="HGNC:HGNC:964"
/db_xref="InterPro:IPR003819"
/db_xref="InterPro:IPR010376"
/db_xref="InterPro:IPR012775"
/db_xref="InterPro:IPR038492"
/db_xref="InterPro:IPR042098"
/db_xref="PDB:3MS5"
/db_xref="PDB:3N6W"
/db_xref="PDB:3O2G"
/db_xref="PDB:4BG1"
/db_xref="PDB:4BGK"
/db_xref="PDB:4BGM"
/db_xref="PDB:4BHF"
/db_xref="PDB:4BHG"
/db_xref="PDB:4BHI"
/db_xref="PDB:4C5W"
/db_xref="PDB:4C8R"
/db_xref="PDB:4CWD"
/db_xref="UniProtKB/Swiss-Prot:O75936"
/protein_id="CAG33093.1"
/translation="MACTIQKAEALDGAHLMQILWYDEEESLYPAVWLRDNCPCSDCY
LDSAKARKLLVEALDVNIGIKGLIFDRKKVYITWPDEHYSEFQADWLKKRCFSKQARA
KLQRELFFPECQYWGSELQLPTLDFEDVLRYDEHAYKWLSTLKKVGIVRLTGASDKPG
EVSKLGKRMGFLYLTFYGHTWQVQDKIDANNVAYTTGKLSFHTDYPALHHPPGVQLLH
CIKQTVTGGDSEIVDGFNVCQKLKKNNPQAFQILSSTFVDFTDIGVDYCDFSVQSKHK
IIELDDKGQVVRINFNNATRDTIFDVPVERVQPFYAALKEFVDLMNSKESKFTFKMNP
GDVITFDNWRLLHGRRSYEAGTEISRHLEGAYADWDVVMSRLRILRQRVENGN"
BASE COUNT 354 a 231 c 269 g 310 t
ORIGIN
1 atggcttgta ccatccaaaa ggcagaagca cttgacgggg ctcatttgat gcagatcctc
61 tggtatgatg aggaagagtc tctctaccca gctgtatggt tgagagacaa ctgtccgtgc
121 tctgattgct acctggattc tgcaaaagca cggaaacttc tagtggaagc tcttgatgtg
181 aacattggaa ttaaaggctt gatatttgac agaaaaaagg tgtacatcac atggcccgat
241 gagcattaca gtgaattcca ggctgattgg ctgaagaaaa gatgcttttc caagcaggcc
301 agagcaaagc tccaaagaga attgtttttt ccagaatgcc aatactgggg ctcagagctc
361 cagctaccca ctttggattt tgaagatgtt ttaagatatg atgaacacgc atacaagtgg
421 ctctccaccc tcaagaaagt aggcatagta agactcaccg gagcatctga caaaccagga
481 gaagtttcaa aacttgggaa aaggatgggt ttcctctatc tcacatttta tggacatact
541 tggcaagtgc aagacaaaat cgatgcaaac aatgtggctt acacaactgg gaagctaagc
601 tttcacactg attatccagc cctccatcat ccacccgggg ttcagcttct tcactgcata
661 aagcaaacag tcacaggggg tgattcagaa attgtagatg ggtttaatgt gtgccaaaaa
721 ctaaagaaaa ataatcctca ggcattccag attttgtcct ctacctttgt ggactttaca
781 gacattggag tggattactg tgatttttct gtacaatcaa aacataaaat tatagagtta
841 gatgataaag gccaagtggt tcgcatcaac ttcaataacg caactaggga cacaatattt
901 gatgtgcctg ttgaaagagt tcagcctttt tatgctgctc tgaaggagtt tgttgacctc
961 atgaacagca aagaatccaa gtttaccttc aagatgaatc caggtgatgt gattactttt
1021 gataactggc gcttacttca tggccgacgt agctatgaag caggaactga gatatcccgc
1081 catctagaag gagcttatgc tgactgggat gtggtcatgt caaggcttcg tatcttaagg
1141 cagagggtgg agaatggaaa ttaa
//