LOCUS       CR456810                 957 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834D1218D for
            gene SGCB, sarcoglycan, beta (43kDa dystrophin-associated
            glycoprotein); complete cds, incl. stopcodon.
ACCESSION   CR456810
VERSION     CR456810.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 957)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834D1218D, ORFNo 1006
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1218D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC (ATG).
            The last base of the last coding triplett has been changed to T,
            which might lead to an amino acid change at the C terminus of the
            polypeptide.
            The stop codon has been set to TAA followed by
            TTAACCCAGCTTTCTT..att.
            Compared to the reference sequence NM_000232 we found amino acid
            exchange(s) at position (first base of changed triplet):
            304(phe->leu)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..957
                     /db_xref="H-InvDB:HIT000267660"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834D1218D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..957
                     /codon_start=1
                     /gene="SGCB"
                     /db_xref="GOA:Q6IBJ4"
                     /db_xref="H-InvDB:HIT000267660.13"
                     /db_xref="InterPro:IPR006875"
                     /db_xref="InterPro:IPR027659"
                     /db_xref="UniProtKB/TrEMBL:Q6IBJ4"
                     /protein_id="CAG33091.1"
                     /translation="MAAAAAAAAEQQSSNGPVKKSMREKAVERRSVNKEHNSNFKAGY
                     IPIDEDRLHKTGLRGRKGNLAICVIILLFILAVINLIITLVIWAVIRIGPNGCDSMEL
                     HESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKT
                     SITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNI
                     KVDGRAIVRGNEGVFIMGKTIEFHMGGNMELKAENSIILNGSVMVSTTRLPSSSSGDQ
                     LGSGDWVRYKLCMCADGTLFKVQVTSQNMGCQISDNPCGNTH"
BASE COUNT          294 a          185 c          236 g          242 t
ORIGIN      
        1 atggcggcag cggcggcggc ggctgcagaa cagcaaagtt ccaatggtcc tgtaaagaag
       61 tccatgcgtg agaaggctgt tgagagaagg agtgtcaata aagagcacaa cagtaacttt
      121 aaagctggat acattccgat tgatgaagat cgtctccaca aaacagggtt gagaggaaga
      181 aagggcaatt tagccatctg tgtgattatc ctcttgttta tcctggctgt catcaattta
      241 ataataacac ttgttatttg ggccgtgatt cgcattggac caaatggctg tgatagtatg
      301 gagcttcatg aaagtggcct gcttcgattt aagcaagtat ctgacatggg agtgatccac
      361 cctctttata aaagcacagt aggaggaagg cgaaatgaaa atttggtcat cactggcaac
      421 aaccagccta ttgtttttca gcaagggaca acaaagctca gtgtagaaaa caacaaaact
      481 tctattacaa gtgacatcgg catgcagttt tttgacccga ggactcaaaa tatcttattc
      541 agcacagact atgaaactca tgagtttcat ttgccaagtg gagtgaaaag tttgaatgtt
      601 caaaaggcat ctactgaaag gattaccagc aatgctacca gtgatttaaa tataaaagtt
      661 gatgggcgtg ctattgtgcg tggaaatgaa ggtgtattca ttatgggcaa aaccattgaa
      721 tttcacatgg gtggtaatat ggagttaaag gcggaaaaca gtatcatcct aaatggatct
      781 gtgatggtca gcaccacccg cctacccagt tcctccagtg gagaccagtt gggtagtggt
      841 gactgggtac gctacaagct ctgcatgtgt gctgatggga cgctcttcaa ggtgcaagta
      901 accagccaga acatgggctg ccaaatctca gacaacccct gtggaaacac tcattaa
//