LOCUS CR456810 957 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1218D for gene SGCB, sarcoglycan, beta (43kDa dystrophin-associated glycoprotein); complete cds, incl. stopcodon. ACCESSION CR456810 VERSION CR456810.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 957) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 957) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D1218D, ORFNo 1006 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1218D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_000232 we found amino acid exchange(s) at position (first base of changed triplet): 304(phe->leu) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..957 /db_xref="H-InvDB:HIT000267660" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D1218D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..957 /codon_start=1 /gene="SGCB" /db_xref="GOA:Q6IBJ4" /db_xref="H-InvDB:HIT000267660.13" /db_xref="InterPro:IPR006875" /db_xref="InterPro:IPR027659" /db_xref="UniProtKB/TrEMBL:Q6IBJ4" /protein_id="CAG33091.1" /translation="MAAAAAAAAEQQSSNGPVKKSMREKAVERRSVNKEHNSNFKAGY IPIDEDRLHKTGLRGRKGNLAICVIILLFILAVINLIITLVIWAVIRIGPNGCDSMEL HESGLLRFKQVSDMGVIHPLYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVENNKT SITSDIGMQFFDPRTQNILFSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNI KVDGRAIVRGNEGVFIMGKTIEFHMGGNMELKAENSIILNGSVMVSTTRLPSSSSGDQ LGSGDWVRYKLCMCADGTLFKVQVTSQNMGCQISDNPCGNTH" BASE COUNT 294 a 185 c 236 g 242 t ORIGIN 1 atggcggcag cggcggcggc ggctgcagaa cagcaaagtt ccaatggtcc tgtaaagaag 61 tccatgcgtg agaaggctgt tgagagaagg agtgtcaata aagagcacaa cagtaacttt 121 aaagctggat acattccgat tgatgaagat cgtctccaca aaacagggtt gagaggaaga 181 aagggcaatt tagccatctg tgtgattatc ctcttgttta tcctggctgt catcaattta 241 ataataacac ttgttatttg ggccgtgatt cgcattggac caaatggctg tgatagtatg 301 gagcttcatg aaagtggcct gcttcgattt aagcaagtat ctgacatggg agtgatccac 361 cctctttata aaagcacagt aggaggaagg cgaaatgaaa atttggtcat cactggcaac 421 aaccagccta ttgtttttca gcaagggaca acaaagctca gtgtagaaaa caacaaaact 481 tctattacaa gtgacatcgg catgcagttt tttgacccga ggactcaaaa tatcttattc 541 agcacagact atgaaactca tgagtttcat ttgccaagtg gagtgaaaag tttgaatgtt 601 caaaaggcat ctactgaaag gattaccagc aatgctacca gtgatttaaa tataaaagtt 661 gatgggcgtg ctattgtgcg tggaaatgaa ggtgtattca ttatgggcaa aaccattgaa 721 tttcacatgg gtggtaatat ggagttaaag gcggaaaaca gtatcatcct aaatggatct 781 gtgatggtca gcaccacccg cctacccagt tcctccagtg gagaccagtt gggtagtggt 841 gactgggtac gctacaagct ctgcatgtgt gctgatggga cgctcttcaa ggtgcaagta 901 accagccaga acatgggctg ccaaatctca gacaacccct gtggaaacac tcattaa //