LOCUS CR456809 819 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0619D for
gene IGFBP5, insulin-like growth factor binding protein 5; complete
cds, incl. stopcodon.
ACCESSION CR456809
VERSION CR456809.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 819)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 819)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0619D, ORFNo 1005
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0619D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC011453 we found amino acid
exchange(s) at position (first base of changed triplet):
814(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..819
/db_xref="H-InvDB:HIT000267659"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0619D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..819
/codon_start=1
/gene="IGFBP5"
/db_xref="H-InvDB:HIT000267659.12"
/protein_id="CAG33090.1"
/translation="MVLLTAVLLLLAAYAGPAQSLGSFVHCEPCDEKALSMCPPSPLG
CELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLN
EKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKL
TQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPN
CDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVD"
BASE COUNT 170 a 269 c 253 g 127 t
ORIGIN
1 atggtgttgc tcaccgcggt cctcctgctg ctggccgcct atgcggggcc ggcccagagc
61 ctgggctcct tcgtgcactg cgagccctgc gacgagaaag ccctctccat gtgccccccc
121 agccccctgg gctgcgagct ggtcaaggag ccgggctgcg gctgctgcat gacctgcgcc
181 ctggccgagg ggcagtcgtg cggcgtctac accgagcgct gcgcccaggg gctgcgctgc
241 ctcccccggc aggacgagga gaagccgctg cacgccctgc tgcacggccg cggggtttgc
301 ctcaacgaaa agagctaccg cgagcaagtc aagatcgaga gagactcccg tgagcacgag
361 gagcccacca cctctgagat ggccgaggag acctactccc ccaagatctt ccggcccaaa
421 cacacccgca tctccgagct gaaggctgaa gcagtgaaga aggaccgcag aaagaagctg
481 acccagtcca agtttgtcgg gggagccgag aacactgccc acccccggat catctctgca
541 cctgagatga gacaggagtc tgagcagggc ccctgccgca gacacatgga ggcttccctg
601 caggagctca aagccagccc acgcatggtg ccccgtgctg tgtacctgcc caattgtgac
661 cgcaaaggat tctacaagag aaagcagtgc aaaccttccc gtggccgcaa gcgtggcatc
721 tgctggtgcg tggacaagta cgggatgaag ctgccaggca tggagtacgt tgacggggac
781 tttcagtgcc acaccttcga cagcagcaac gttgattaa
//