LOCUS CR456795 1602 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C1021D for
gene PHGDH, phosphoglycerate dehydrogenase; complete cds, incl.
stopcodon.
ACCESSION CR456795
VERSION CR456795.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1602)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1602)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C1021D, ORFNo 954
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C1021D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_006623 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1602
/db_xref="H-InvDB:HIT000267645"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C1021D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1602
/codon_start=1
/gene="PHGDH"
/db_xref="GOA:O43175"
/db_xref="H-InvDB:HIT000267645.13"
/db_xref="HGNC:HGNC:8923"
/db_xref="InterPro:IPR006139"
/db_xref="InterPro:IPR006140"
/db_xref="InterPro:IPR006236"
/db_xref="InterPro:IPR029009"
/db_xref="InterPro:IPR029752"
/db_xref="InterPro:IPR029753"
/db_xref="InterPro:IPR036291"
/db_xref="PDB:2G76"
/db_xref="PDB:5N53"
/db_xref="PDB:5N6C"
/db_xref="PDB:5NZO"
/db_xref="PDB:5NZP"
/db_xref="PDB:5NZQ"
/db_xref="PDB:5OFM"
/db_xref="PDB:5OFV"
/db_xref="PDB:5OFW"
/db_xref="UniProtKB/Swiss-Prot:O43175"
/protein_id="CAG33076.1"
/translation="MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAE
LQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGN
SLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREV
ATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLN
DNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHE
NVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIG
LAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVN
AKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNG
AVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHV
MGISSLLPSLEAWKQHVTEAFQFHF"
BASE COUNT 338 a 438 c 500 g 326 t
ORIGIN
1 atggcttttg caaatctgcg gaaagtgctc atcagtgaca gcctggaccc ttgctgccgg
61 aagatcttgc aagatggagg gctgcaggtg gtggaaaagc agaaccttag caaagaggag
121 ctgatagcgg agctgcagga ctgtgaaggc cttattgttc gctctgccac caaggtgacc
181 gctgatgtca tcaacgcagc tgagaaactc caggtggtgg gcagggctgg cacaggtgtg
241 gacaatgtgg atctggaggc cgcaacaagg aagggcatct tggttatgaa cacccccaat
301 gggaacagcc tcagtgccgc agaactcact tgtggaatga tcatgtgcct ggccaggcag
361 attccccagg cgacggcttc gatgaaggac ggcaaatggg agcggaagaa gttcatggga
421 acagagctga atggaaagac cctgggaatt cttggcctgg gcaggattgg gagagaggta
481 gctacccgga tgcagtcctt tgggatgaag actatagggt atgaccccat catttcccca
541 gaggtctcgg cctcctttgg tgttcagcag ctgcccctgg aggagatctg gcctctctgt
601 gatttcatca ctgtgcacac tcctctcctg ccctccacga caggcttgct gaatgacaac
661 acctttgccc agtgcaagaa gggggtgcgt gtggtgaact gtgcccgtgg agggatcgtg
721 gacgaaggcg ccctgctccg ggccctgcag tctggccagt gtgccggggc tgcactggac
781 gtgtttacgg aagagccgcc acgggaccgg gccttggtgg accatgagaa tgtcatcagc
841 tgtccccacc tgggtgccag caccaaggag gctcagagcc gctgtgggga ggaaattgct
901 gttcagttcg tggacatggt gaaggggaaa tctctcacgg gggttgtgaa tgcccaggcc
961 cttaccagtg ccttctctcc acacaccaag ccttggattg gtctggcaga agctctgggg
1021 acactgatgc gagcctgggc tgggtccccc aaagggacca tccaggtgat aacacaggga
1081 acatccctga agaatgctgg gaactgccta agccccgcag tcattgtcgg cctcctgaaa
1141 gaggcttcca agcaggcgga tgtgaacttg gtgaacgcta agctgctggt gaaagaggct
1201 ggcctcaatg tcaccacctc ccacagccct gctgcaccag gggagcaagg cttcggggaa
1261 tgcctcctgg ccgtggccct ggcaggcgcc ccttaccagg ctgtgggctt ggtccaaggc
1321 actacacctg tactgcaggg gctcaatgga gctgtcttca ggccagaagt gcctctccgc
1381 agggacctgc ccctgctcct attccggact cagacctctg accctgcaat gctgcctacc
1441 atgattggcc tcctggcaga ggcaggcgtg cggctgctgt cctaccagac ttcactggtg
1501 tcagatgggg agacctggca cgtcatgggc atctcctcct tgctgcccag cctggaagcg
1561 tggaagcagc atgtgactga agccttccag ttccactttt aa
//