LOCUS CR456787 1443 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G094D for
gene UMPS, uridine monophosphate synthetase (orotate phosphoribosyl
transferase and orotidine-5'-decarboxylase); complete cds, incl.
stopcodon.
ACCESSION CR456787
VERSION CR456787.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1443)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1443)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G094D, ORFNo 941
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G094D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_000373 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1443
/db_xref="H-InvDB:HIT000267637"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G094D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1443
/codon_start=1
/gene="UMPS"
/db_xref="GOA:P11172"
/db_xref="H-InvDB:HIT000267637.13"
/db_xref="HGNC:HGNC:12563"
/db_xref="InterPro:IPR000836"
/db_xref="InterPro:IPR001754"
/db_xref="InterPro:IPR004467"
/db_xref="InterPro:IPR011060"
/db_xref="InterPro:IPR013785"
/db_xref="InterPro:IPR014732"
/db_xref="InterPro:IPR018089"
/db_xref="InterPro:IPR023031"
/db_xref="InterPro:IPR029057"
/db_xref="PDB:2EAW"
/db_xref="PDB:2JGY"
/db_xref="PDB:2P1F"
/db_xref="PDB:2QCC"
/db_xref="PDB:2QCD"
/db_xref="PDB:2QCE"
/db_xref="PDB:2QCF"
/db_xref="PDB:2QCG"
/db_xref="PDB:2QCH"
/db_xref="PDB:2QCL"
/db_xref="PDB:2QCM"
/db_xref="PDB:2QCN"
/db_xref="PDB:2V30"
/db_xref="PDB:2WNS"
/db_xref="PDB:3BGG"
/db_xref="PDB:3BGJ"
/db_xref="PDB:3BK0"
/db_xref="PDB:3BVJ"
/db_xref="PDB:3DBP"
/db_xref="PDB:3EWU"
/db_xref="PDB:3EWW"
/db_xref="PDB:3EWX"
/db_xref="PDB:3EWY"
/db_xref="PDB:3EWZ"
/db_xref="PDB:3EX1"
/db_xref="PDB:3EX2"
/db_xref="PDB:3EX3"
/db_xref="PDB:3EX4"
/db_xref="PDB:3EX6"
/db_xref="PDB:3G3D"
/db_xref="PDB:3G3M"
/db_xref="PDB:3L0K"
/db_xref="PDB:3L0N"
/db_xref="PDB:3MI2"
/db_xref="PDB:3MO7"
/db_xref="PDB:3MW7"
/db_xref="PDB:4HIB"
/db_xref="PDB:4HKP"
/db_xref="UniProtKB/Swiss-Prot:P11172"
/protein_id="CAG33068.1"
/translation="MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIV
SRPRLLSQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKETKD
YGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAIVLLDREQGGK
DKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRFIQENVFVAANHNGSPLSI
KEAPKELSFGARAELPRIHPVASKLLRLMQKKETNLCLSADVSLARELLQLADALGPS
ICMLKTHVDILNDFTLDVMKELITLAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIA
SWADLVNAHVVPGSGVVKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEE
HSEFVVGFISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVG
RGIISAADRLEAAEMYRKAAWEAYLSRLGV"
BASE COUNT 381 a 288 c 393 g 381 t
ORIGIN
1 atggcggtcg ctcgtgcagc tttggggcca ttggtgacgg gtctgtacga cgtgcaggct
61 ttcaagtttg gggacttcgt gctgaagagc gggctttcct cccccatcta catcgatctg
121 cggggcatcg tgtctcgacc gcgtcttctg agtcaggttg cagatatttt attccaaact
181 gcccaaaatg caggcatcag ttttgacacc gtgtgtggag tgccttatac agctttgcca
241 ttggctacag ttatctgttc aaccaatcaa attccaatgc ttattagaag gaaagaaaca
301 aaggattatg gaactaagcg tcttgtagaa ggaactatta atccaggaga aacctgttta
361 atcattgaag atgttgtcac cagtggatct agtgttttgg aaactgttga ggttcttcag
421 aaggagggct tgaaggtcac tgatgccata gtgctgttgg acagagagca gggaggcaag
481 gacaagttgc aggcgcacgg gatccgcctc cactcagtgt gtacattgtc caaaatgctg
541 gagattctcg agcagcagaa aaaagttgat gctgagacag ttgggagagt gaagaggttt
601 attcaggaga atgtctttgt ggcagcgaat cataatggtt ctcccctttc tataaaggaa
661 gcacccaaag aactcagctt cggtgcacgt gcagagctgc ccaggatcca cccagttgca
721 tcgaagcttc tcaggcttat gcaaaagaag gagaccaatc tgtgtctatc tgctgatgtt
781 tcactggcca gagagctgtt gcagctagca gatgctttag gacctagtat ctgcatgctg
841 aagactcatg tagatatttt gaatgatttt actctggatg tgatgaagga gttgataact
901 ctggcaaaat gccatgagtt cttgatattt gaagaccgga agtttgcaga tataggaaac
961 acagtgaaaa agcagtatga aggaggtatc tttaaaatag cttcctgggc agatctagta
1021 aatgctcacg tggtgccagg ctcaggagtt gtgaaaggcc tgcaagaagt gggcctgcct
1081 ttgcatcggg ggtgcctcct tattgcggaa atgagctcca ccggctccct ggccactggg
1141 gactacacta gagcagcggt tagaatggct gaggagcact ctgaatttgt tgttggtttt
1201 atttctggct cccgagtaag catgaaacca gaatttcttc acttgactcc aggagttcag
1261 ttggaagcag gaggagataa tcttggccaa cagtacaata gcccacaaga agttattggc
1321 aaacgaggtt ccgatatcat cattgtaggt cgtggcataa tctcagcagc tgatcgtctg
1381 gaagcagcag agatgtacag aaaagctgct tgggaagcgt atttgagtag acttggtgtt
1441 taa
//