LOCUS CR456783 1068 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G034D for
gene GNAI2, guanine nucleotide binding protein (G protein), alpha
inhibiting activity polypeptide 2; complete cds, incl. stopcodon.
ACCESSION CR456783
VERSION CR456783.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1068)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1068)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G034D, ORFNo 932
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G034D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC014627 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1068
/db_xref="H-InvDB:HIT000267633"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G034D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1068
/codon_start=1
/gene="GNAI2"
/db_xref="GOA:Q96C71"
/db_xref="H-InvDB:HIT000267633.14"
/db_xref="InterPro:IPR001019"
/db_xref="InterPro:IPR001408"
/db_xref="InterPro:IPR011025"
/db_xref="InterPro:IPR027417"
/db_xref="UniProtKB/TrEMBL:Q96C71"
/protein_id="CAG33064.1"
/translation="MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAVES
GKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRAD
DARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDL
ERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEG
VTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFE
EKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQ
FVFDAVTDVIIKNNLKDCGLF"
BASE COUNT 271 a 294 c 303 g 200 t
ORIGIN
1 atgggctgca ccgtgagcgc cgaggacaag gcggcggccg agcgctctaa gatgatcgac
61 aagaacctgc gggaggacgg agagaaggcg gcgcgggagg tgaagttgct gctgttgggt
121 gctgtggagt cagggaagag caccatcgtc aagcagatga agatcatcca cgaggatggc
181 tactccgagg aggaatgccg gcagtaccgg gcggttgtct acagcaacac catccagtcc
241 atcatggcca ttgtcaaagc catgggcaac ctgcagatcg actttgccga cccctccaga
301 gcggacgacg ccaggcagct atttgcactg tcctgcaccg ccgaggagca aggcgtgctc
361 cctgatgacc tgtccggcgt catccggagg ctctgggctg accatggtgt gcaggcctgc
421 tttggccgct caagggaata ccagctcaac gactcagctg cctactacct gaacgacctg
481 gagcgtattg cacagagtga ctacatcccc acacagcaag atgtgctacg gacccgcgta
541 aagaccacgg ggatcgtgga gacacacttc accttcaagg acctacactt caagatgttt
601 gatgtgggtg gtcagcggtc tgagcggaag aagtggatcc actgctttga gggcgtcaca
661 gccatcatct tctgcgtagc cttgagcgcc tatgacttgg tgctagctga ggacgaggag
721 atgaaccgca tgcatgagag catgaagcta ttcgatagca tctgcaacaa caagtggttc
781 acagacacgt ccatcatcct cttcctcaac aagaaggacc tgtttgagga gaagatcaca
841 cacagtcccc tgaccatctg cttccctgag tacacagggg ccaacaaata tgatgaggca
901 gccagctaca tccagagtaa gtttgaggac ctgaataagc gcaaagacac caaggagatc
961 tacacgcact tcacgtgcgc caccgacacc aagaacgtgc agttcgtgtt tgacgccgtc
1021 accgatgtca tcatcaagaa caacctgaag gactgcggcc tcttttaa
//