LOCUS CR456773 747 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E054D for gene RPL7, ribosomal protein L7; complete cds, incl. stopcodon. ACCESSION CR456773 VERSION CR456773.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 747) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 747) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834E054D, ORFNo 905 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E054D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_000971 we found amino acid exchange(s) at position (first base of changed triplet): 142(lys->glu) 574(his->arg) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..747 /db_xref="H-InvDB:HIT000267623" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834E054D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..747 /codon_start=1 /gene="RPL7" /db_xref="GOA:P18124" /db_xref="H-InvDB:HIT000267623.12" /db_xref="HGNC:HGNC:10363" /db_xref="InterPro:IPR005998" /db_xref="InterPro:IPR012988" /db_xref="InterPro:IPR016082" /db_xref="InterPro:IPR018038" /db_xref="InterPro:IPR023106" /db_xref="InterPro:IPR035808" /db_xref="InterPro:IPR036919" /db_xref="InterPro:IPR039699" /db_xref="PDB:4UG0" /db_xref="PDB:4V6X" /db_xref="PDB:5AJ0" /db_xref="PDB:5LKS" /db_xref="PDB:5T2C" /db_xref="PDB:6EK0" /db_xref="PDB:6IP5" /db_xref="PDB:6IP6" /db_xref="PDB:6IP8" /db_xref="PDB:6OLE" /db_xref="PDB:6OLF" /db_xref="PDB:6OLG" /db_xref="PDB:6OLI" /db_xref="PDB:6OLZ" /db_xref="PDB:6OM0" /db_xref="PDB:6OM7" /db_xref="PDB:6QZP" /db_xref="UniProtKB/Swiss-Prot:P18124" /protein_id="CAG33054.1" /translation="MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRK ARRELIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVS PKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYG KINKKRIALTDNALIARSLGKYGIICMEDLIREIYTVGKRFKEANNFLWPFKLSSPRG GMKKKTTHFVEGGDAGNREDQINRLIRRMN" BASE COUNT 249 a 134 c 190 g 174 t ORIGIN 1 atggagggtg tagaagagaa gaagaaggag gttcctgctg tgccagaaac ccttaagaaa 61 aagcgaagga atttcgcaga gctgaagatc aagcgcctga gaaagaagtt tgcccaaaag 121 atgcttcgaa aggcaaggag ggagcttatc tatgaaaaag caaagcacta tcacaaggaa 181 tataggcaga tgtacagaac tgaaattcga atggcgagga tggcaagaaa agctggcaac 241 ttctatgtac ctgcagaacc caaattggcg tttgtcatca gaatcagagg tatcaatgga 301 gtgagcccaa aggttcgaaa ggtgttgcag cttcttcgcc ttcgtcaaat cttcaatgga 361 acctttgtga agctcaacaa ggcttcgatt aacatgctga ggattgtaga gccatatatt 421 gcatgggggt accccaatct gaagtcagta aatgaactaa tctacaagcg tggttatggc 481 aaaatcaata agaagcgaat tgctttgaca gataacgctt tgattgctcg atctcttggt 541 aaatacggca tcatctgcat ggaggatttg attcgtgaga tctatactgt tggaaaacgc 601 ttcaaagagg caaataactt cctgtggccc ttcaaattgt cttctccacg aggtggaatg 661 aagaaaaaga ccacccattt tgtagaaggt ggagatgctg gcaacaggga ggaccagatc 721 aacaggctta ttagaagaat gaattaa //