LOCUS CR456730 1269 bp mRNA linear HUM 21-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0114D for
gene NOL4, nucleolar protein 4; complete cds, incl. stopcodon.
ACCESSION CR456730
VERSION CR456730.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1269)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1269)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F0114D, ORFNo 768
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0114D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC000313 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1269
/db_xref="H-InvDB:HIT000267580"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F0114D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1269
/codon_start=1
/gene="NOL4"
/db_xref="GOA:O94818"
/db_xref="H-InvDB:HIT000267580.12"
/db_xref="HGNC:HGNC:7870"
/db_xref="InterPro:IPR026747"
/db_xref="InterPro:IPR039788"
/db_xref="UniProtKB/Swiss-Prot:O94818"
/protein_id="CAG33011.1"
/translation="MHVETGPNGEQIRKHAGQKRTYKAISESYAFLPREAVTRFLMSC
SECQKRMHLNPDGTDHKDNGKPPTLVTSMIDYNMPITMAYMKHMKLQLLNSQQDEDES
SIESDEFDMSDSTRMSAVNSDLSSNLEERMQSPQNLHGQQDDDSAAESFNGNETLGHS
SIASGGTHSREMGDSNSDGKTGLEQDEQPLNLSDSPLSAQLTSEYRIDDHNSNGKNKY
KNLLISDLKMEREARENGSKSPAHSYSSYDSGKNESVDRGAEDLSLNRGDEDEDDHED
HDDSEKVNETDGVEAERLKAFNDESAPADKQCKPEATQATYSTSAVPGSQDVLYINGN
GTYSYHSYRGLGGGLLNLNDASSSGPTDLSMKRQLATSSGSSSSSNSRPQLSPTEINA
VRQLVAGYRESAAFLLRSADELENLILQQN"
BASE COUNT 423 a 273 c 312 g 261 t
ORIGIN
1 atgcatgtgg aaacggggcc aaatggagaa caaattcgga aacacgctgg acaaaagaga
61 acttacaaag caatttcaga gagctatgcc ttcctaccaa gagaagcggt gacacgattt
121 ctaatgagct gctcagagtg ccagaaaaga atgcatttaa acccagatgg aacagatcat
181 aaagataatg gaaaacctcc cactttggtg accagcatga ttgactacaa catgccaatt
241 accatggcct acatgaaaca catgaagctg cagctgctaa actcacagca agatgaggat
301 gaaagttcaa tagaaagtga tgaatttgac atgagtgatt caacacggat gtcagctgtg
361 aactctgatc ttagctccaa tcttgaagaa agaatgcaaa gtccccagaa tcttcatggc
421 cagcaagatg atgattctgc tgcagagagc tttaatggca atgagactct ggggcacagt
481 tcaattgctt cagggggaac acacagcagg gagatgggag actccaacag tgatggcaaa
541 actgggctgg agcaagatga acagccactg aacctgagtg acagtcccct ctctgcgcag
601 ctaacttcgg aatacagaat agatgatcac aacagtaatg ggaaaaacaa gtataagaat
661 cttctaattt ctgacctcaa gatggaacga gaggcgagag aaaatggaag caagtctcct
721 gcacatagtt actccagcta tgactctggc aaaaatgaga gtgtagaccg aggagctgag
781 gacctctcac taaacagggg agatgaggac gaagatgacc acgaggacca tgacgattcg
841 gagaaagtta atgagacaga cggcgttgaa gccgagcggc tgaaagcttt taatgatgag
901 tctgctccag ctgacaaaca gtgtaaacca gaggcgaccc aggccactta ctcaacatca
961 gctgttccag gctcacagga cgtgctgtac atcaatggaa atgggaccta tagttaccat
1021 agttacagag ggctaggagg gggtctgcta aatctgaatg atgcttccag cagtggaccc
1081 actgatctca gcatgaagag acaattggcg actagctcag gatcctccag cagctcaaac
1141 tccagacccc agctgagtcc aactgaaatc aatgccgtga gacagcttgt tgcaggatat
1201 cgagaatcag ctgcattttt attgcgatct gcagatgaac tggaaaatct cattttacaa
1261 cagaattaa
//