LOCUS CR456728 1902 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H1014D for
gene XRCC1, X-ray repair complementing defective repair in Chinese
hamster cells 1; complete cds, incl. stopcodon.
ACCESSION CR456728
VERSION CR456728.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1902)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1902)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H1014D, ORFNo 758
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H1014D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_006297 we found amino acid
exchange(s) at position (first base of changed triplet):
1195(arg->gln)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1902
/db_xref="H-InvDB:HIT000267578"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H1014D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1902
/codon_start=1
/gene="XRCC1"
/db_xref="GOA:P18887"
/db_xref="H-InvDB:HIT000267578.13"
/db_xref="HGNC:HGNC:12828"
/db_xref="InterPro:IPR001357"
/db_xref="InterPro:IPR002706"
/db_xref="InterPro:IPR008979"
/db_xref="InterPro:IPR036420"
/db_xref="PDB:1CDZ"
/db_xref="PDB:1XNA"
/db_xref="PDB:1XNT"
/db_xref="PDB:2D8M"
/db_xref="PDB:2W3O"
/db_xref="PDB:3K75"
/db_xref="PDB:3K77"
/db_xref="PDB:3LQC"
/db_xref="PDB:5E6Q"
/db_xref="PDB:5W7X"
/db_xref="PDB:5W7Y"
/db_xref="UniProtKB/Swiss-Prot:P18887"
/protein_id="CAG33009.1"
/translation="MPEIRLRHVVSCSSQDSTHCAENLLKADTYRKWRAAKAGEKTIS
VVLQLEKEEQIHSVDIGNDGSAFVEVLVGSSAGGAGEQDYEVLLVTSSFMSPSESRSG
SNPNRVRMFGPDKLVRAAAEKRWDRVKIVCSQPYSKDSPFGLSFVRFHSPPDKDEAEA
PSQKVTVTKLGQFRVKEEDESANSLRPGALFFSRINKTSPVTASDPAGPSYAAATLQA
SSAASSASPVSRAIGSTSKPQESPKGKRKLDLNQEEKKTPSKPPAQLSPSVPKRPKLP
APTRTPATAPVPARAQGAVTGKPRGEGTEPRRPRAGPEELGKILQGVVVVLSGFQNPF
RSELRDKALELGAKYRPDWTRDSTHLICAFANTPKYSQVLGLGGRIVRKEWVLDCHRM
RRRLPSQRYLMAGPGSSSEEDEASHSGGSGDEAPKLPQKQPQTKTKPTQAAGPSSPQK
PPTPEETKAASPVLQEDIDIEGVQSEGQDNGAEDSGDTEDELRRVAEQKEHRLPPGQE
ENGEDPYAGSTDENTDSEEHQEPPDLPVPELPDFFQGKHFFLYGEFPGDERRKLIRYV
TAFNGELEDYMSDRVQFVITAQEWDPSFEEALMDNPSLAFVRPRWIYSCNEKQKLLPH
QLYGVVPQA"
BASE COUNT 455 a 564 c 559 g 324 t
ORIGIN
1 atgccggaga tccgcctccg ccatgtcgtg tcctgcagca gccaggactc gactcactgt
61 gcagaaaatc ttctcaaggc agacacttac cgaaaatggc gggcagccaa ggcaggcgag
121 aagaccatct ctgtggtcct acagttggag aaggaggagc agatacacag tgtggacatt
181 gggaatgatg gctcagcttt cgtggaggtg ctggtgggca gttcagctgg aggcgctggg
241 gagcaagact atgaggtcct tctggtcacc tcatctttca tgtccccttc cgagagccgc
301 agtggctcaa accccaaccg cgttcgcatg tttgggcctg acaagctggt ccgggcagcc
361 gccgagaagc gctgggaccg ggtcaaaatt gtttgcagcc agccctacag caaggactcc
421 ccctttggct tgagttttgt acggtttcat agccccccag acaaagatga ggcagaggcc
481 ccgtcccaga aggtgacagt gaccaagctt ggccagttcc gtgtgaagga ggaggatgag
541 agcgccaact ctctgaggcc gggggctctc ttcttcagcc ggatcaacaa gacatcccca
601 gtcacagcca gcgacccagc aggacctagc tatgcagctg ctaccctcca ggcttctagt
661 gctgcctcct cagcctctcc agtctccagg gccataggca gcacctccaa gccccaggag
721 tctcccaaag ggaagaggaa gttggatttg aaccaagaag aaaagaagac ccccagcaaa
781 ccaccagccc agctgtcgcc atctgttccc aagagaccta aattgccagc tccaactcgt
841 accccagcca cagccccagt ccctgcccga gcacaggggg cagtgacagg caaaccccga
901 ggagaaggca ccgagcccag acgaccccga gctggcccag aggagctggg gaagatcctt
961 cagggtgtgg tagtggtgct gagtggcttc cagaacccct tccgctccga gctgcgagat
1021 aaggccctag agcttggggc caagtatcgg ccagactgga cccgggacag cacgcacctc
1081 atctgtgcct ttgccaacac ccccaagtac agccaggtcc taggcctggg aggccgcatc
1141 gtgcgtaagg agtgggtgct ggactgtcac cgcatgcgtc ggcggctgcc ctcccagagg
1201 tacctcatgg cagggccagg ttccagcagt gaggaggatg aggcctctca cagcggtggc
1261 agcggagatg aagcccccaa gcttcctcag aagcaacccc agaccaaaac caagcccact
1321 caggcagctg gacccagctc accccagaag cccccaaccc ctgaagagac caaagcagcc
1381 tcaccagtgc tccaggaaga tatagacatt gagggggtac agtcagaagg acaggacaat
1441 ggggcggaag attctgggga cacagaggat gagctgagga gggtggcaga gcagaaggaa
1501 cacagactgc cccctggcca ggaggagaat ggggaagacc cgtatgcagg ctccacggat
1561 gagaacacgg acagtgagga acaccaggag cctcctgatc tgccagtccc tgagctccca
1621 gatttcttcc agggcaagca cttctttctt tacggggagt tccctgggga cgagcggcgg
1681 aaactcatcc gatacgtcac agccttcaat ggggagctcg aggactatat gagtgaccgg
1741 gttcagtttg tgatcacagc acaggaatgg gatcccagct ttgaggaggc cctgatggac
1801 aacccctccc tggcattcgt tcgtccccga tggatctaca gttgcaatga gaagcagaag
1861 ttacttcctc accagctcta tggggtggtg ccgcaagctt aa
//