LOCUS CR456723 786 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H103D for
gene HADH2, hydroxyacyl-Coenzyme A dehydrogenase, type II; complete
cds, incl. stopcodon.
ACCESSION CR456723
VERSION CR456723.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 786)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 786)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H103D, ORFNo 744
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H103D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_004493 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..786
/db_xref="H-InvDB:HIT000267573"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H103D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..786
/codon_start=1
/gene="HADH2"
/db_xref="GOA:Q99714"
/db_xref="H-InvDB:HIT000267573.12"
/db_xref="HGNC:HGNC:4800"
/db_xref="InterPro:IPR002347"
/db_xref="InterPro:IPR020904"
/db_xref="InterPro:IPR036291"
/db_xref="PDB:1F67"
/db_xref="PDB:1SO8"
/db_xref="PDB:1U7T"
/db_xref="PDB:2O23"
/db_xref="UniProtKB/Swiss-Prot:Q99714"
/protein_id="CAG33004.1"
/translation="MAAACRSVKGLVAVITGGASGLGLATAERLVGQGASAVLLDLPN
SGGEAQAKKLGNNCVFAPADVTSEKDVQTALALAKGKFGRVDVAVNCAGIAVASKTYN
LKKGQTHTLEDFQRVLDVNLMGTFNVIRLVAGEMGQNEPDQGGQRGVIINTASVAAFE
GQVGQAAYSASKGGIVGMTLPIARDLAPIGIRVMTIAPGLFGTPLLTSLPEKVCNFLA
SQVPFPSRLGDPAEYAHLVQAIIENPFLNGEVIRLDGAIRMQP"
BASE COUNT 169 a 212 c 245 g 160 t
ORIGIN
1 atggcagcag cgtgtcggag cgtgaagggc ctggtggcgg taataaccgg aggagcctcg
61 ggcctgggcc tggccacggc ggagcgactt gtggggcagg gagcctctgc tgtgcttctg
121 gacctgccca actcgggtgg ggaggcccaa gccaagaagt taggaaacaa ctgcgttttc
181 gccccagccg acgtgacctc tgagaaggat gtgcaaacag ctctggctct agcaaaagga
241 aagtttggcc gtgtggatgt agctgtcaac tgtgcaggca tcgcggtggc tagcaagacg
301 tacaacttaa agaagggcca gacccatacc ttggaagact tccagcgagt tcttgatgtg
361 aatctcatgg gcaccttcaa tgtgatccgc ctggtggctg gtgagatggg ccagaatgaa
421 ccagaccagg gaggccaacg tggggtcatc atcaacactg ccagtgtggc tgccttcgag
481 ggccaggttg gacaagctgc atactctgct tccaaggggg gaatagtggg catgacactg
541 cccattgctc gggatctggc tcccataggt atccgggtga tgaccattgc cccaggtctg
601 tttggcaccc cactgctgac cagcctccca gagaaagtgt gcaacttctt ggccagccaa
661 gtgcccttcc ctagccgact gggtgaccct gctgagtatg ctcacctcgt acaggccatc
721 atcgagaacc cattcctcaa tggagaggtc atccggctgg atggggccat tcgtatgcag
781 ccttaa
//