LOCUS CR456713 1044 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G073D for
gene SOX5, SRY (sex determining region Y)-box 5; complete cds,
incl. stopcodon.
ACCESSION CR456713
VERSION CR456713.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1044)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1044)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G073D, ORFNo 712
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G073D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence S83308 we found amino acid
exchange(s) at position (first base of changed triplet):
124(lys->glu) 292(gln->glu)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1044
/db_xref="H-InvDB:HIT000267563"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G073D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1044
/codon_start=1
/gene="SOX5"
/db_xref="GOA:Q6IBT9"
/db_xref="H-InvDB:HIT000267563.13"
/db_xref="InterPro:IPR009071"
/db_xref="InterPro:IPR036910"
/db_xref="UniProtKB/TrEMBL:Q6IBT9"
/protein_id="CAG32994.1"
/translation="MPALRINSGAGPLKASVPAALASPSARVSTIGYLNDHDAVTEAI
QEARQMKEQLRREQQVLDGKVAVVNSLGLNNCRTEKEKTTLESLTQQLAVKQNEEGKF
SHAMMDFNLSGDSDGSAGVSESRIYRESRGRGSNEPHIKRPMNAFMVWAKDERRKILQ
AFPDMHNSNISKILGSRWKAMTNLEKQPYYEEQARLSKQHLEKYPDYKYKPRPKRTCL
VDGKKLRIGEYKAIMRNRRQEMRQYFNVGQQAQIPIATAGVVYPGAIAMAGMPSPHLP
SEHSSVSSSPEPGMPVIQSTYGVKGEEPHIKEEIQAEDINGEIYDEYDEEEDDPDVDY
GSDSENHIAGQAN"
BASE COUNT 326 a 237 c 279 g 202 t
ORIGIN
1 atgccagctc tgagaataaa cagtggggca ggccccctca aagcctctgt cccagcagcg
61 ttagctagtc cttcagccag agttagcaca ataggttact taaatgacca tgatgctgtc
121 accgaggcaa tccaagaagc tcggcaaatg aaggagcaac tccgacggga acaacaggtg
181 cttgatggga aggtggctgt tgtgaatagt ctgggtctca ataactgccg aacagaaaag
241 gaaaaaacaa cactggagag tctgactcag caactggcag ttaaacagaa tgaagaagga
301 aaatttagcc atgcaatgat ggatttcaat ctgagtggag attctgatgg aagtgctgga
361 gtctcagagt caagaattta tagggaatcc cgagggcgtg gtagcaatga accccacata
421 aagcgtccaa tgaatgcctt catggtgtgg gctaaagatg aacggagaaa gatccttcaa
481 gcctttcctg acatgcacaa ctccaacatc agcaagatat tgggatctcg ctggaaagct
541 atgacaaacc tagagaaaca gccatattat gaggagcaag cccgtctcag caagcagcac
601 ctggagaagt accctgacta taagtacaag cccaggccaa agcgcacctg cctggtggat
661 ggcaaaaagc tgcgcattgg tgaatacaag gcaatcatgc gcaacaggcg gcaggaaatg
721 cggcagtact tcaatgttgg gcaacaagca cagatcccca ttgccactgc tggtgttgtg
781 taccctggag ccatcgccat ggctgggatg ccctcccctc acctgccctc ggagcactca
841 agcgtgtcta gcagcccaga gcctgggatg cctgttatcc agagcactta cggtgtgaaa
901 ggagaggagc cacatatcaa agaagagata caggccgagg acatcaatgg agaaatttat
961 gatgagtacg acgaggaaga ggatgatcca gatgtagatt atgggagtga cagtgaaaac
1021 catattgcag gacaagccaa ttaa
//