LOCUS CR456702 1425 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E083D for
gene RNF14, ring finger protein 14; complete cds, incl. stopcodon.
ACCESSION CR456702
VERSION CR456702.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1425)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1425)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E083D, ORFNo 646
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E083D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_183401 we found amino acid
exchange(s) at position (first base of changed triplet):
94(gln->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1425
/db_xref="H-InvDB:HIT000267552"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E083D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1425
/codon_start=1
/gene="RNF14"
/db_xref="GOA:Q9UBS8"
/db_xref="H-InvDB:HIT000267552.13"
/db_xref="HGNC:HGNC:10058"
/db_xref="InterPro:IPR001841"
/db_xref="InterPro:IPR002867"
/db_xref="InterPro:IPR006575"
/db_xref="InterPro:IPR013083"
/db_xref="InterPro:IPR016135"
/db_xref="InterPro:IPR017907"
/db_xref="InterPro:IPR031127"
/db_xref="UniProtKB/Swiss-Prot:Q9UBS8"
/protein_id="CAG32983.1"
/translation="MSSEDREAQEDELLALASIYDGDEFRKAESVRGGETRIYLDLPQ
NFKIFVSGNSNECLQNSGFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQ
LSALCKHLDNLWEEHRGSVVLFAWMQFLKEETLAYLNIVSPFELKIGSQKKVQRRTAQ
ASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQAQQIKCFNSKLF
LCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATP
GQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNF
AFCTLCRLTYHGVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEME
SKEWLEKNSKSCPCCGTPIEKLDGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPG
SPCFNRLFYAVDVDDDIWEDEVED"
BASE COUNT 387 a 295 c 360 g 383 t
ORIGIN
1 atgtcgtcag aagatcgaga agctcaggag gatgaattgc tggccctggc aagtatttac
61 gatggagatg aatttagaaa agcagagtct gtccgaggtg gagaaaccag gatctatttg
121 gatttgccac agaatttcaa gatatttgtg agcggcaatt caaatgagtg tctccagaat
181 agtggctttg aatacaccat ttgctttctg cctccacttg tgctgaactt tgaactgcca
241 ccagattatc catcctcttc cccaccttca ttcacactta gtggcaaatg gctgtcacca
301 actcagctat ctgctctatg caagcactta gacaacctat gggaagaaca ccgtggcagc
361 gtggtcctgt ttgcctggat gcaatttctt aaggaagaga ccctagcata cttgaatatt
421 gtctctcctt ttgagctcaa gattggttct cagaaaaaag tgcagagaag gacagctcaa
481 gcttctccca acacagagct agattttgga ggagctgctg gatctgatgt agaccaagag
541 gaaattgtgg atgagagagc agtgcaggat gtggaatcac tgtcaaatct gatccaggaa
601 atcttggact ttgatcaagc tcagcagata aaatgcttta atagtaaatt gttcctgtgc
661 agtatctgtt tctgtgagaa gctgggtagt gaatgcatgt acttcttgga gtgcaggcat
721 gtgtactgca aagcctgtct gaaggactac tttgaaatcc agatcagaga tggccaggtt
781 caatgcctca actgcccaga accaaagtgc ccttcggtgg ccactcctgg tcaggtcaaa
841 gagttagtgg aagcagagtt atttgcccgt tatgaccgcc ttctcctcca gtcctccttg
901 gacctgatgg cagatgtggt gtactgcccc cggccgtgct gccagctgcc tgtgatgcag
961 gaacctggct gcaccatggg tatctgctcc agctgcaatt ttgccttctg tactttgtgc
1021 aggttgacct accatggggt ctccccatgt aaggtgactg cagagaaatt aatggactta
1081 cgaaatgaat acctgcaagc ggatgaggct aataaaagac ttttggatca aaggtatggt
1141 aagagagtga ttcagaaggc actggaagag atggaaagta aggagtggct agagaagaac
1201 tcaaagagct gcccatgttg tggaactccc atagagaaat tagacggatg taacaagatg
1261 acatgtactg gctgtatgca atatttctgt tggatttgca tgggttctct ctctagagca
1321 aacccttaca aacatttcaa tgaccctggt tcaccatgtt ttaaccggct gttttatgct
1381 gtggatgttg acgacgatat ttgggaagat gaggtagaag attaa
//