LOCUS CR450358 309 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C013D for
gene MGP, matrix Gla protein; complete cds; without stopcodon.
ACCESSION CR450358
VERSION CR450358.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 309)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 309)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C013D, ORFNo 582
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C013D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_000900 we found amino acid
exchange(s) at position (first base of changed triplet):
151(arg->gly)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..309
/db_xref="H-InvDB:HIT000267267"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C013D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>309
/codon_start=1
/gene="MGP"
/db_xref="GOA:P08493"
/db_xref="H-InvDB:HIT000267267.11"
/db_xref="HGNC:HGNC:7060"
/db_xref="InterPro:IPR000294"
/db_xref="InterPro:IPR002384"
/db_xref="InterPro:IPR027118"
/db_xref="InterPro:IPR035972"
/db_xref="UniProtKB/Swiss-Prot:P08493"
/protein_id="CAG29354.1"
/translation="MKSLILLAILAALAVVTLCYESHESMESYELNPFINRRNANTFI
SPQQRWGAKVQERIRERSKPVHELNREACDDYRLCERYAMVYGYNAAYNRYFRKRRGA
K"
BASE COUNT 90 a 74 c 72 g 73 t
ORIGIN
1 atgaagagcc tgatccttct tgccatcctg gccgccttag cggtagtaac tttgtgttat
61 gaatcacatg aaagcatgga atcttatgaa cttaatccct tcattaacag gagaaatgca
121 aataccttca tatcccctca gcagagatgg ggagctaaag tccaagagag gatccgagaa
181 cgctctaagc ctgtccacga gctcaatagg gaagcctgtg atgactacag actttgcgaa
241 cgctacgcca tggtttatgg atacaatgct gcctataatc gctacttcag gaagcgccga
301 ggggccaaa
//