LOCUS CR450353 1053 bp mRNA linear HUM 17-APR-2005
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H102D for
gene ATF4, activating transcription factor 4 (tax-responsive
enhancer element B67); complete cds; without stopcodon.
ACCESSION CR450353
VERSION CR450353.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1053)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1053)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H102D, ORFNo 525
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H102D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_001675 we found amino acid
exchange(s) at position (first base of changed triplet):
991(ile->thr)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1053
/db_xref="H-InvDB:HIT000267262"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H102D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1053
/codon_start=1
/gene="ATF4"
/db_xref="GOA:Q6ICP0"
/db_xref="H-InvDB:HIT000267262.12"
/db_xref="InterPro:IPR004827"
/db_xref="InterPro:IPR029811"
/db_xref="UniProtKB/TrEMBL:Q6ICP0"
/protein_id="CAG29349.1"
/translation="MTEMSFLSSEVLVGDLMSPFDQSGLGAEESLGLLDDYLEVAKHF
KPHGFSSDKAKAGSSEWLAVDGLVSPSNNSKEDAFSGTDWMLEKMDLKEFDLDALLGI
DDLETMPDDLLTTLDDTCDLFAPLVQETNKQPPQTVNPIGHLPESLTKPDQVAPFTFL
QPLPLSPGVLSSTPDHSFSLELGSEVDITEGDRKPDYTAYVAMIPQCIKEEDTPSDND
SGICMSPESYLGSPQHSPSTRGSPNRSLPSPGVLCGSARPKPYDPPGEKMVAAKVKGE
KLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKERADSLAKETQYL
KDLIEEVRKARGKKRVP"
BASE COUNT 280 a 264 c 290 g 219 t
ORIGIN
1 atgaccgaaa tgagcttcct gagcagcgag gtgttggtgg gggacttgat gtcccccttc
61 gaccagtcgg gtttgggggc tgaagaaagc ctaggtctct tagatgatta cctggaggtg
121 gccaagcact tcaaacctca tgggttctcc agcgacaagg ctaaggcggg ctcctccgaa
181 tggctggctg tggatgggtt ggtcagtccc tccaacaaca gcaaggagga tgccttctcc
241 gggacagatt ggatgttgga gaaaatggat ttgaaggagt tcgacttgga tgccctgttg
301 ggtatagatg acctggaaac catgccagat gaccttctga ccacgttgga tgacacttgt
361 gatctctttg cccccctagt ccaggagact aataagcagc ccccccagac ggtgaaccca
421 attggccatc tcccagaaag tttaacaaaa cctgaccagg ttgccccctt caccttctta
481 caacctcttc ccctttcccc aggggtcctg tcctccactc cagatcattc ctttagttta
541 gagctgggca gtgaagtgga tatcactgaa ggagatagga agccagacta cactgcttac
601 gttgccatga tccctcagtg cataaaggag gaagacaccc cttcagataa tgatagtggc
661 atctgtatga gcccagagtc ctatctgggg tctcctcagc acagcccctc taccaggggc
721 tctccaaata ggagcctccc atctccaggt gttctctgtg ggtctgcccg tcccaaacct
781 tacgatcctc ctggagagaa gatggtagca gcaaaagtaa agggtgagaa actggataag
841 aagctgaaaa aaatggagca aaacaagaca gcagccacta ggtaccgcca gaagaagagg
901 gcggagcagg aggctcttac tggtgagtgc aaagagctgg aaaagaagaa cgaggctcta
961 aaagagaggg cggattccct ggccaaggag acccagtacc tgaaagattt gatagaagag
1021 gtccgcaagg caagggggaa gaaaagggtc ccc
//