LOCUS CR450350 720 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H032D for
gene SNRPN, small nuclear ribonucleoprotein polypeptide N; complete
cds; without stopcodon.
ACCESSION CR450350
VERSION CR450350.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 720)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 720)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H032D, ORFNo 509
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H032D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_022808 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..720
/db_xref="H-InvDB:HIT000267259"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H032D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>720
/codon_start=1
/gene="SNRPN"
/db_xref="GOA:P63162"
/db_xref="H-InvDB:HIT000267259.13"
/db_xref="HGNC:HGNC:11164"
/db_xref="InterPro:IPR001163"
/db_xref="InterPro:IPR010920"
/db_xref="InterPro:IPR017131"
/db_xref="PDB:5MF9"
/db_xref="UniProtKB/Swiss-Prot:P63162"
/protein_id="CAG29346.1"
/translation="MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCD
CDEFRKIKPKNAKQPEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAG
GPGVGRAAGRGVPAGVPIPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAVAATAS
IAGAPTQYPPGRGTPPPPVGRATPPPGIMAPPPGMRPPMGPPIGLPPARGTPIGMPPP
GMRPPPPGIRGPPPPGMRPPRP"
BASE COUNT 165 a 203 c 208 g 144 t
ORIGIN
1 atgactgttg gcaagagtag caagatgctg cagcacattg actatagaat gagatgtatc
61 ctgcaagatg gccgaatctt cattggcacc tttaaggctt ttgacaagca tatgaatttg
121 atcctctgtg attgtgatga gttcagaaag atcaagccaa agaatgcgaa gcaaccagag
181 cgtgaagaaa agcgggtttt gggtctggtg ttgctgcgtg gggagaactt ggtatccatg
241 actgtggagg ggccaccccc caaagatact ggcattgctc gggtaccact tgctggagct
301 gctggaggcc ctggggttgg tagggcagct ggtagaggag taccagctgg tgtgccaatt
361 ccccaggccc ctgctggatt ggcaggccct gtccgaggag ttgggggacc atcccagcag
421 gtaatgactc cacagggaag aggcactgta gcagctgctg ctgttgctgc gactgccagt
481 attgctggag ccccaacaca gtacccacca ggacggggca ctccgccccc acccgtcggc
541 agagcaaccc cacctccagg cattatggct cctccacctg gtatgagacc acccatgggc
601 ccaccaattg ggcttccccc tgctcgaggg acgccaatag gcatgccgcc tccgggaatg
661 agaccccctc caccaggcat tagaggtcca cctcccccag gaatgcgtcc accaagacct
//