LOCUS CR450341 579 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D102D for
gene AP3S1, adaptor-related protein complex 3, sigma 1 subunit;
complete cds; without stopcodon.
ACCESSION CR450341
VERSION CR450341.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 579)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 579)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D102D, ORFNo 417
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D102D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_001284 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..579
/db_xref="H-InvDB:HIT000267250"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D102D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>579
/codon_start=1
/gene="AP3S1"
/db_xref="GOA:Q92572"
/db_xref="H-InvDB:HIT000267250.11"
/db_xref="HGNC:HGNC:2013"
/db_xref="InterPro:IPR000804"
/db_xref="InterPro:IPR011012"
/db_xref="InterPro:IPR016635"
/db_xref="InterPro:IPR022775"
/db_xref="UniProtKB/Swiss-Prot:Q92572"
/protein_id="CAG29337.1"
/translation="MIKAILIFNNHGKPRLSKFYQPYSEDTQQQIIRETFHLVSKRDE
NVCNFLEGGLLIGGSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFE
NVCELDLIFHVDKVHNILAEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPAR
AVSAVKNMNLPEIPRNINIGDISIKVPNLPSFK"
BASE COUNT 190 a 98 c 124 g 167 t
ORIGIN
1 atgatcaagg cgatcctaat cttcaacaac cacgggaagc cgcggctctc caagttctac
61 cagccctaca gtgaagatac acaacagcaa atcatcaggg agactttcca tttggtatct
121 aagagagatg aaaatgtttg taatttccta gaaggaggat tattaattgg aggatctgac
181 aacaaactga tttatagaca ttatgcaacg ttatattttg tcttctgtgt ggattcttca
241 gaaagtgaac ttggcatttt agatctaatt caagtatttg tggaaacatt agacaaatgt
301 tttgaaaatg tctgtgagct ggatttgatt ttccatgtag acaaggttca caatattctt
361 gcagaaatgg tgatgggggg aatggtattg gagacaaata tgaatgagat tgttacacaa
421 attgatgcac aaaataagct ggaaaaatct gaggctggct tagcaggagc tccagcccgt
481 gctgtatcag ctgtaaagaa tatgaatctt cctgagatcc caagaaatat taacattggt
541 gacatcagta taaaagtgcc aaacctgccc tcttttaaa
//