LOCUS CR450339 342 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C112D for
gene RPLP1, ribosomal protein, large, P1; complete cds; without
stopcodon.
ACCESSION CR450339
VERSION CR450339.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 342)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 342)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C112D, ORFNo 395
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C112D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_001003 we found amino acid
exchange(s) at position (first base of changed triplet):
43(ile->val)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..342
/db_xref="H-InvDB:HIT000267248"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C112D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>342
/codon_start=1
/gene="RPLP1"
/db_xref="GOA:Q6ICQ4"
/db_xref="H-InvDB:HIT000267248.13"
/db_xref="InterPro:IPR027534"
/db_xref="InterPro:IPR038716"
/db_xref="UniProtKB/TrEMBL:Q6ICQ4"
/protein_id="CAG29335.1"
/translation="MASVSELACIYSALVLHDDEVTVTEDKINALIKAAGVNVEPFWP
GLFAKALANVNIGSLICNVGAGGPAPAAGAAPAGGPAPSTAAAPAEEKKVEAKKEESE
ESDDDMGFGLFD"
BASE COUNT 75 a 90 c 99 g 78 t
ORIGIN
1 atggcctctg tctccgagct cgcctgcatc tactcggccc tcgttctgca cgacgatgag
61 gtgacagtca cggaggataa gatcaatgcc ctcattaaag cagccggtgt aaatgttgag
121 cctttttggc ctggcttgtt tgcaaaggcc ctggccaacg tcaacattgg gagcctcatc
181 tgcaatgtag gggccggtgg acctgctcca gcagctggtg ctgcaccagc aggaggtcct
241 gccccctcca ctgctgctgc tccagctgag gagaagaaag tggaagcaaa gaaagaagaa
301 tccgaggagt ctgatgatga catgggcttt ggtctttttg ac
//