LOCUS CR450335 573 bp mRNA linear HUM 18-MAY-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E016D for
gene ARHG, ras homolog gene family, member G (rho G); complete cds;
without stopcodon.
ACCESSION CR450335
VERSION CR450335.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 573)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 573)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E016D, ORFNo 254
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E016D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_001665 we found amino acid
exchange(s) at position (first base of changed triplet):
397(ser->gly)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..573
/db_xref="H-InvDB:HIT000267244"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E016D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>573
/codon_start=1
/gene="ARHG"
/db_xref="GOA:Q6ICQ8"
/db_xref="H-InvDB:HIT000267244.12"
/db_xref="InterPro:IPR001806"
/db_xref="InterPro:IPR003578"
/db_xref="InterPro:IPR005225"
/db_xref="InterPro:IPR027417"
/db_xref="UniProtKB/TrEMBL:Q6ICQ8"
/protein_id="CAG29331.1"
/translation="MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQS
AVDGRTVNLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVC
HHCPDVPILLVGTKKDLRAQPDTLRRLKEQGQAPITPQQGQALAKQIHAVRYLECSAL
QQDGVKEVFAEAVRAVLNPTPIKRGRSCILL"
BASE COUNT 119 a 184 c 165 g 105 t
ORIGIN
1 atgcagagca tcaagtgcgt ggtggtgggt gatggggctg tgggcaagac gtgcctgctc
61 atctgctaca caactaacgc tttccccaaa gagtacatcc ccaccgtgtt cgacaattac
121 agcgcgcaga gcgcagttga cgggcgcaca gtgaacctga acctgtggga cactgcgggc
181 caggaggagt atgaccgcct ccgtacactc tcctaccctc agaccaacgt tttcgtcatc
241 tgtttctcca ttgccagtcc gccgtcctat gagaacgtgc ggcacaagtg gcatccagag
301 gtgtgccacc actgccctga tgtgcccatc ctgctggtgg gcaccaagaa ggacctgaga
361 gcccagcctg acaccctacg gcgcctcaag gagcagggcc aggcgcccat cacaccgcag
421 cagggccagg cactggccaa gcagatccac gctgtgcgct acctcgaatg ctcagccctg
481 caacaggatg gtgtcaagga agtgttcgcc gaggctgtcc gggctgtgct caaccccacg
541 ccgatcaagc gtgggcggtc ctgcatcctc ttg
//