LOCUS CR450321 1131 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G031D for
gene PEX14, peroxisomal biogenesis factor 14; complete cds; without
stopcodon.
ACCESSION CR450321
VERSION CR450321.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1131)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1131)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G031D, ORFNo 174
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G031D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_004565 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1131
/db_xref="H-InvDB:HIT000267230"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G031D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1131
/codon_start=1
/gene="PEX14"
/db_xref="GOA:O75381"
/db_xref="H-InvDB:HIT000267230.13"
/db_xref="HGNC:HGNC:8856"
/db_xref="InterPro:IPR006785"
/db_xref="InterPro:IPR025655"
/db_xref="InterPro:IPR036388"
/db_xref="PDB:2W84"
/db_xref="PDB:2W85"
/db_xref="PDB:4BXU"
/db_xref="UniProtKB/Swiss-Prot:O75381"
/protein_id="CAG29317.1"
/translation="MASSEQAEQPSQPSSTPGSENVLPREPLIATAVKFLQNSRVRQS
PLATRRAFLKKKGLTDEEIDMAFQQSGTAADEPSSLGPATQVVPVQPPHLISQPYSPA
GSRWRDYGALAIIMAGIAFGFHQLYKKYLLPLILGGREDRKQLERMEAGLSELSGSVA
QTVTQLQTTLASVQELLIQQQQKIQELAHELAAAKATTSTNWILESQNINELKSEINS
LKGLLLNRRQFPPSPSAPKIPSWQIPVKSPSPSSPAAVNHHSSSDISPVSNESTSSSP
GKEGHSPEGSTVTYHLLGPQEEGEGVVDVKGQVRMEVQGEEEKREDKEDEEDEEDDDV
SHVDEEDCLGVQREDRRGGDGQINEQVEKLRRPEGASNESERD"
BASE COUNT 265 a 335 c 353 g 178 t
ORIGIN
1 atggcgtcct cggagcaggc agagcagccg agccagccaa gctctactcc aggaagtgaa
61 aatgtgctgc ctcgagagcc gctgattgcc acggcagtga agtttctaca gaattcccgg
121 gtccgccaga gcccacttgc aaccaggaga gcatttctaa agaagaaagg gctgacagat
181 gaagagattg atatggcctt ccagcagtcg ggcactgctg ccgatgagcc ttcgtccttg
241 ggcccagcca cacaggtggt tcctgtccag ccccctcacc tcatatctca gccatacagt
301 cccgcaggct cccgatggcg agattacggc gccctggcca tcatcatggc aggcattgca
361 tttggctttc accagctcta caagaaatac ctgctccccc tcatcctggg cggccgagag
421 gacagaaagc agctggagag gatggaggcc ggtctctctg agctgagtgg cagcgtggcc
481 cagacagtga ctcagttaca gacgaccctc gcctccgtcc aggagctgct gattcagcag
541 cagcagaaaa tccaggagct tgcccacgag ctggccgctg ccaaggccac cacatccacc
601 aactggatcc tggagtccca gaatatcaac gaactcaagt ccgaaattaa ctccttgaaa
661 gggcttcttt taaatcggag gcagttccct ccatccccat cagccccgaa gatcccctcc
721 tggcagatcc cagtcaagtc accgtcaccc tccagccctg cggccgtgaa ccaccacagc
781 agcagcgaca tctcacctgt cagcaacgag tccacgtcgt cctcgcctgg gaaggagggc
841 cacagccccg agggctccac ggtcacctac cacttgctgg gcccccagga ggaaggcgag
901 ggggtggtgg acgtcaaggg ccaggtgcgg atggaggtgc aaggcgagga ggagaagagg
961 gaggacaagg aggacgagga ggatgaggag gatgatgatg tgagccatgt ggacgaggag
1021 gactgcctgg gggtgcagag ggaggaccgc cggggcgggg atgggcagat caacgagcag
1081 gtggagaagc tgcggcggcc cgagggcgcc agcaacgaga gtgagcggga c
//