LOCUS CR450312 318 bp mRNA linear HUM 18-MAY-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F061D for
gene GLRX, glutaredoxin (thioltransferase); complete cds; without
stopcodon.
ACCESSION CR450312
VERSION CR450312.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 318)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 318)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F061D, ORFNo 159
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F061D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_002064 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..318
/db_xref="H-InvDB:HIT000267221"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F061D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>318
/codon_start=1
/gene="GLRX"
/db_xref="GOA:P35754"
/db_xref="H-InvDB:HIT000267221.13"
/db_xref="HGNC:HGNC:4330"
/db_xref="InterPro:IPR002109"
/db_xref="InterPro:IPR011767"
/db_xref="InterPro:IPR011899"
/db_xref="InterPro:IPR014025"
/db_xref="InterPro:IPR036249"
/db_xref="PDB:1B4Q"
/db_xref="PDB:1JHB"
/db_xref="PDB:4RQR"
/db_xref="UniProtKB/Swiss-Prot:P35754"
/protein_id="CAG29308.1"
/translation="MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLE
FVDITATNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLKQI
GALQ"
BASE COUNT 87 a 74 c 84 g 73 t
ORIGIN
1 atggctcaag agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag
61 cccacctgcc cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa
121 gggcttctgg aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat
181 ttgcaacagc tcacgggagc aagaacggtg cctcgagtct ttattggtaa agattgtata
241 ggcggatgca gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag
301 cagattggag ctctgcag
//