LOCUS CR450299 354 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C111D for gene MLANA, melan-A; complete cds; without stopcodon. ACCESSION CR450299 VERSION CR450299.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 354) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 354) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834C111D, ORFNo 97 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C111D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Ina Rolfs RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 101 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon (ATG): att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG) After the last codon additional sequence has been added: CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC TT). Compared to the reference sequence NM_005511 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..354 /db_xref="H-InvDB:HIT000267208" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834C111D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>354 /codon_start=1 /gene="MLANA" /db_xref="GOA:Q16655" /db_xref="H-InvDB:HIT000267208.11" /db_xref="HGNC:HGNC:7124" /db_xref="InterPro:IPR029242" /db_xref="PDB:2GT9" /db_xref="PDB:2GTW" /db_xref="PDB:2GTZ" /db_xref="PDB:2GUO" /db_xref="PDB:3HG1" /db_xref="PDB:3L6F" /db_xref="PDB:3MRO" /db_xref="PDB:3MRP" /db_xref="PDB:3MRQ" /db_xref="PDB:3QDM" /db_xref="PDB:3QEQ" /db_xref="PDB:3QFD" /db_xref="PDB:4EUP" /db_xref="PDB:4JFF" /db_xref="PDB:4L3E" /db_xref="PDB:4QOK" /db_xref="PDB:4WJ5" /db_xref="PDB:5E9D" /db_xref="PDB:5NHT" /db_xref="PDB:5NQK" /db_xref="PDB:6D78" /db_xref="PDB:6DKP" /db_xref="UniProtKB/Swiss-Prot:Q16655" /protein_id="CAG29295.1" /translation="MPREDAHFIYGYPKKGHGHSYTTAEEAAGIGILTVILGVLLLIG CWYCRRRNGYRALMDKSLHVGTQCALTRRCPQEGFDHRDSKVSLQEKNCEPVVPNAPP AYEKLSAEQSPPPYSP" BASE COUNT 99 a 88 c 84 g 83 t ORIGIN 1 atgccaagag aagatgctca cttcatctat ggttacccca agaaggggca cggccactct 61 tacaccacgg ctgaagaggc cgctgggatc ggcatcctga cagtgatcct gggagtctta 121 ctgctcatcg gctgttggta ttgtagaaga cgaaatggat acagagcctt gatggataaa 181 agtcttcatg ttggcactca atgtgcctta acaagaagat gcccacaaga agggtttgat 241 catcgggaca gcaaagtgtc tcttcaagag aaaaactgtg aacctgtggt tcccaatgct 301 ccacctgctt atgagaaact ctctgcagaa cagtcaccac caccttattc acct //