LOCUS CR450291 807 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B081D for
gene ELA2A, elastase 2A; complete cds; without stopcodon.
ACCESSION CR450291
VERSION CR450291.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 807)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 807)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B081D, ORFNo 69
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B081D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_033440 we found amino acid
exchange(s) at position (first base of changed triplet):
592(thr->ala)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..807
/db_xref="H-InvDB:HIT000267200"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B081D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>807
/codon_start=1
/gene="ELA2A"
/db_xref="GOA:Q6ICV2"
/db_xref="H-InvDB:HIT000267200.13"
/db_xref="InterPro:IPR001254"
/db_xref="InterPro:IPR001314"
/db_xref="InterPro:IPR009003"
/db_xref="InterPro:IPR018114"
/db_xref="InterPro:IPR033116"
/db_xref="UniProtKB/TrEMBL:Q6ICV2"
/protein_id="CAG29287.1"
/translation="MIRTLLLSTLVAGALSCGDPTYPPYVTRVVGGEEARPNSWPWQV
SLQYSSNGKWYHTCGGSLIANSWVLTAAHCISSSRTYRVGLGRHNLYVAESGSLAVSV
SKIVVHKDWNSNQISKGNDIALLKLANPVSLTDKIQLACLPPAGTILPNNYPCYVTGW
GRLQTNGAVPDVLQQGRLLVVDYATCSSSAWWGSSVKASMICAGGDGVISSCNGDSGG
PLNCQASDGRWQVHGIVSFGSRLGCNYYHKPSVFTRVSNYIDWINSVIANN"
BASE COUNT 156 a 254 c 233 g 164 t
ORIGIN
1 atgataagga cgctgctgct gtccactttg gtggctggag ccctcagttg tggggacccc
61 acttacccac cttatgtgac tagggtggtt ggcggtgaag aagcgaggcc caacagctgg
121 ccctggcagg tctccctgca gtacagctcc aatggcaagt ggtaccacac ctgcggaggg
181 tccctgatag ccaacagctg ggtcctgacg gctgcccact gcatcagctc ctccaggacc
241 taccgcgtgg ggctgggccg gcacaacctc tacgttgcgg agtccggctc gctggcagtc
301 agtgtctcta agattgtggt gcacaaggac tggaactcca accaaatctc caaagggaac
361 gacattgccc tgctcaaact ggctaacccc gtctccctca ccgacaagat ccagctggcc
421 tgcctccctc ctgccggcac cattctaccc aacaactacc cctgctacgt cacgggctgg
481 ggaaggctgc agaccaacgg ggctgttcct gatgtcctgc agcagggccg gttgctggtt
541 gtggactatg ccacctgctc cagctctgcc tggtggggca gcagcgtgaa agccagtatg
601 atctgtgctg ggggtgatgg cgtgatctcc agctgcaacg gagactctgg cgggccactg
661 aactgtcagg catctgacgg ccggtggcag gtgcacggca tcgtcagctt cgggtcccgc
721 ctcggctgca actactacca caagccctcc gtcttcacgc gggtctccaa ttacatcgac
781 tggatcaatt cggtgattgc aaataac
//