LOCUS CR450283 267 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A051D for gene CXCL12, chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); complete cds; without stopcodon. ACCESSION CR450283 VERSION CR450283.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 267) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 267) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (18-MAY-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834A051D, ORFNo 48 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A051D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Ina Rolfs RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 101 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon (ATG): att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG) After the last codon additional sequence has been added: CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC TT). Compared to the reference sequence NM_199168 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..267 /db_xref="H-InvDB:HIT000267192" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834A051D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>267 /codon_start=1 /gene="CXCL12" /db_xref="GOA:P48061" /db_xref="H-InvDB:HIT000267192.14" /db_xref="HGNC:HGNC:10672" /db_xref="InterPro:IPR001811" /db_xref="InterPro:IPR033899" /db_xref="InterPro:IPR036048" /db_xref="InterPro:IPR039813" /db_xref="PDB:1A15" /db_xref="PDB:1QG7" /db_xref="PDB:1SDF" /db_xref="PDB:1VMC" /db_xref="PDB:2J7Z" /db_xref="PDB:2K01" /db_xref="PDB:2K03" /db_xref="PDB:2K04" /db_xref="PDB:2K05" /db_xref="PDB:2KEC" /db_xref="PDB:2KED" /db_xref="PDB:2KEE" /db_xref="PDB:2KOL" /db_xref="PDB:2N55" /db_xref="PDB:2NWG" /db_xref="PDB:2SDF" /db_xref="PDB:3GV3" /db_xref="PDB:3HP3" /db_xref="PDB:4LMQ" /db_xref="PDB:4UAI" /db_xref="UniProtKB/Swiss-Prot:P48061" /protein_id="CAG29279.1" /translation="MNAKVVVVLVLVLTALCLSDGKPVSLSYRCPCRFFESHVARANV KHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK" BASE COUNT 73 a 75 c 66 g 53 t ORIGIN 1 atgaacgcca aggtcgtggt cgtgctggtc ctcgtgctga ccgcgctctg cctcagcgac 61 gggaagcccg tcagcctgag ctacagatgc ccatgccgat tcttcgaaag ccatgttgcc 121 agagccaacg tcaagcatct caaaattctc aacactccaa actgtgccct tcagattgta 181 gcccggctga agaacaacaa cagacaagtg tgcattgacc cgaagctaaa gtggattcag 241 gagtacctgg agaaagcttt aaacaag //