LOCUS CR407661 384 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A033D for
gene CD59, CD59 antigen p18-20 (antigen identified by monoclonal
antibodies 16.3A5, EJ16, EJ30, EL32 and G344) complete cds, without
stopcodon.
ACCESSION CR407661
VERSION CR407661.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 384)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 384)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A033D, ORFNo 536
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A033D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_000611 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..384
/db_xref="H-InvDB:HIT000267158"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A033D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>384
/codon_start=1
/gene="CD59"
/db_xref="GOA:Q6FHM9"
/db_xref="H-InvDB:HIT000267158.11"
/db_xref="InterPro:IPR016054"
/db_xref="InterPro:IPR018363"
/db_xref="InterPro:IPR027101"
/db_xref="UniProtKB/TrEMBL:Q6FHM9"
/protein_id="CAG28589.1"
/translation="MGIQGGSVLFGLLLVLAVFCHSGHSLQCYNCPNPTADCKTAVNC
SSDFDACLITKAGLQVYNKCWKFEHCNFNDVTTRLRENELTYYCCKKDLCNFNEQLEN
GGTSLSEKTVLLLVTPFLAAAWSLHP"
BASE COUNT 96 a 93 c 91 g 104 t
ORIGIN
1 atgggaatcc aaggagggtc tgtcctgttc gggctgctgc tcgtcctggc tgtcttctgc
61 cattcaggtc atagcctgca gtgctacaac tgtcctaacc caactgctga ctgcaaaaca
121 gccgtcaatt gttcatctga ttttgatgcg tgtctcatta ccaaagctgg gttacaagtg
181 tataacaagt gttggaagtt tgagcattgc aatttcaacg acgtcacaac ccgcttgagg
241 gaaaatgagc taacgtacta ctgctgcaag aaggacctgt gtaactttaa cgaacagctt
301 gaaaatggtg ggacatcctt atcagagaaa acagttcttc tgctggtgac tccatttctg
361 gcagcagcct ggagccttca tccc
//