LOCUS       CR407657                 858 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834H092D for
            gene CAPZA1, capping protein (actin filament) muscle Z-line, alpha
            1 complete cds, without stopcodon.
ACCESSION   CR407657
VERSION     CR407657.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 858)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
            D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834H092D, ORFNo 523
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H092D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Ina Rolfs
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 101
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon
            (ATG):
            att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
            After the last codon additional sequence has been added:
            CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
            TT).
            Compared to the reference sequence BC000144 we did not find any
            amino acid exchanges.
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..858
                     /db_xref="H-InvDB:HIT000267154"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834H092D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>858
                     /codon_start=1
                     /gene="CAPZA1"
                     /db_xref="GOA:P52907"
                     /db_xref="H-InvDB:HIT000267154.13"
                     /db_xref="HGNC:HGNC:1488"
                     /db_xref="InterPro:IPR002189"
                     /db_xref="InterPro:IPR017865"
                     /db_xref="InterPro:IPR037282"
                     /db_xref="InterPro:IPR042276"
                     /db_xref="InterPro:IPR042489"
                     /db_xref="PDB:1MQ1"
                     /db_xref="PDB:1MWN"
                     /db_xref="UniProtKB/Swiss-Prot:P52907"
                     /protein_id="CAG28585.1"
                     /translation="MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDN
                     LLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLR
                     KEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIES
                     HQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTV
                     SNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILS
                     YKIGKEMQNA"
BASE COUNT          279 a          174 c          194 g          211 t
ORIGIN      
        1 atggccgact tcgatgatcg tgtgtcggat gaggagaagg tacgcatagc tgctaaattc
       61 atcactcatg cacccccagg ggaatttaat gaagtattca atgacgttcg gctactactt
      121 aataatgaca atctcctcag ggaaggggca gcacatgcat ttgcccagta taacatggat
      181 cagttcacgc ctgtgaagat agaaggatat gaagatcagg tcttaattac agagcacggt
      241 gacctgggta atagcagatt tttagatcca agaaacaaaa tttcctttaa atttgaccac
      301 ttacggaaag aagcaagtga cccccagcca gaagaagcag atggaggtct gaagtcttgg
      361 agagaatcct gtgacagtgc tttaagagcc tatgtgaaag accattattc caacggcttc
      421 tgtactgttt atgctaaaac tatcgatggg caacagacta ttattgcatg tattgaaagc
      481 caccagtttc agcctaaaaa cttctggaat ggtcgttgga gatcagagtg gaagttcacc
      541 atcacaccac ctacagccca ggtggttggc gtgcttaaga ttcaggttca ctattatgaa
      601 gatggcaatg ttcagttggt tagtcataaa gatgtacagg attcactaac tgtttcgaat
      661 gaagcccaaa ctgccaagga gtttattaaa atcatagaga atgcagaaaa tgagtatcag
      721 acagcaatta gtgaaaacta tcaaacaatg tcagatacca cattcaaggc cttgcgccgg
      781 cagcttccag ttacccgcac caaaatcgac tggaacaaga tactcagcta caagattggc
      841 aaagaaatgc agaatgct
//