LOCUS CR407653 738 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H042D for
gene TFAM, transcription factor A, mitochondrial complete cds,
without stopcodon.
ACCESSION CR407653
VERSION CR407653.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 738)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 738)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H042D, ORFNo 510
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H042D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_003201 we found amino acid
exchange(s) at position (first base of changed triplet):
34(ser->thr) 106(val->ala)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..738
/db_xref="H-InvDB:HIT000267150"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H042D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>738
/codon_start=1
/gene="TFAM"
/db_xref="GOA:Q6LES8"
/db_xref="H-InvDB:HIT000267150.13"
/db_xref="InterPro:IPR009071"
/db_xref="InterPro:IPR036910"
/db_xref="UniProtKB/TrEMBL:Q6LES8"
/protein_id="CAG28581.1"
/translation="MAFLRSMWGVLTALGRSGAELCTGCGSRLRSPFSFAYLPRWFSS
VLASCPKKPVSSYLRFSKEQLPIFKAQNPDAKTTELIRRIAQRWRELPDSKKKIYQDA
YRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRS
AYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELYIQHAKEDETRYHNEMKSW
EEQMIEVGRKDLLRRTIKKQRKYGAEEC"
BASE COUNT 264 a 128 c 170 g 176 t
ORIGIN
1 atggcgtttc tccgaagcat gtggggcgtg ctgactgccc tgggaaggtc tggagcagag
61 ctgtgcaccg gctgtggaag tcgactgcgc tcccccttca gttttgcgta tttaccgagg
121 tggttttcat ctgtcttggc aagttgtcca aagaaacctg taagttctta ccttcgattt
181 tctaaagaac aactacccat atttaaagct cagaacccag atgcaaaaac tacagaacta
241 attagaagaa ttgcccagcg ttggagggaa cttcctgatt caaagaaaaa aatatatcaa
301 gatgcttata gggcggagtg gcaggtatat aaagaagaga taagcagatt taaagaacag
361 ctaactccaa gtcagattat gtctttggaa aaagaaatca tggacaaaca tttaaaaagg
421 aaagctatga caaaaaaaaa agagttaaca ctgcttggaa aaccaaaaag acctcgttca
481 gcttataacg tttatgtagc tgaaagattc caagaagcta agggtgattc accgcaggaa
541 aagctgaaga ctgtaaagga aaactggaaa aatctgtctg actctgaaaa ggaattatat
601 attcagcatg ctaaagagga cgaaactcgt tatcataatg aaatgaagtc ttgggaagaa
661 caaatgattg aagttggacg aaaggatctt ctacgtcgca caataaagaa acaacgaaaa
721 tatggtgctg aggagtgt
//