LOCUS       CR407634                 456 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834E032D for
            gene UBE2B, ubiquitin-conjugating enzyme E2B (RAD6 homolog)
            complete cds, without stopcodon.
ACCESSION   CR407634
VERSION     CR407634.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 456)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
            D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834E032D, ORFNo 434
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E032D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Ina Rolfs
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 101
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon
            (ATG):
            att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
            After the last codon additional sequence has been added:
            CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
            TT).
            Compared to the reference sequence NM_003337 we did not find any
            amino acid exchanges.
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..456
                     /db_xref="H-InvDB:HIT000267131"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834E032D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>456
                     /codon_start=1
                     /gene="UBE2B"
                     /db_xref="GOA:P63146"
                     /db_xref="H-InvDB:HIT000267131.13"
                     /db_xref="HGNC:HGNC:12473"
                     /db_xref="InterPro:IPR000608"
                     /db_xref="InterPro:IPR016135"
                     /db_xref="InterPro:IPR023313"
                     /db_xref="PDB:1JAS"
                     /db_xref="PDB:1NXA"
                     /db_xref="PDB:2Y4W"
                     /db_xref="PDB:2YB6"
                     /db_xref="PDB:2YBF"
                     /db_xref="UniProtKB/Swiss-Prot:P63146"
                     /protein_id="CAG28562.1"
                     /translation="MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPE
                     GTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDV
                     SSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS"
BASE COUNT          145 a           93 c           99 g          119 t
ORIGIN      
        1 atgtcgaccc cggcccggag gaggctcatg cgggatttca agcggttaca agaggaccca
       61 cctgtgggtg tcagtggcgc accatctgaa aacaacatca tgcagtggaa tgcagttata
      121 tttggaccag aagggacacc ttttgaagat ggtactttta aactagtaat agaattttct
      181 gaagaatatc caaataaacc accaactgtt aggtttttat ccaaaatgtt tcatccaaat
      241 gtgtatgctg atggtagcat atgtttagat atccttcaga atcgatggag tccaacatat
      301 gacgtatctt ctatcttaac atcaattcag tctctgctgg atgaaccgaa tcctaacagt
      361 ccagccaata gccaggcagc acagctttat caggaaaaca aacgagaata tgagaaaaga
      421 gtttcggcca ttgttgaaca aagctggaat gattca
//