LOCUS CR407629 465 bp mRNA linear HUM 10-MAY-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D062D for
gene MGST1, microsomal glutathione S-transferase 1 complete cds,
without stopcodon.
ACCESSION CR407629
VERSION CR407629.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 465)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 465)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D062D, ORFNo 412
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D062D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_145764 we found amino acid
exchange(s) at position (first base of changed triplet):
220(arg->lys)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..465
/db_xref="H-InvDB:HIT000267126"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D062D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>465
/codon_start=1
/gene="MGST1"
/db_xref="GOA:Q6LET6"
/db_xref="H-InvDB:HIT000267126.13"
/db_xref="InterPro:IPR001129"
/db_xref="InterPro:IPR023352"
/db_xref="InterPro:IPR040162"
/db_xref="UniProtKB/TrEMBL:Q6LET6"
/protein_id="CAG28557.1"
/translation="MVDLTQVMDDEVFMAFASYATIILSKMMLMSTATAFYRLTRKVF
ANPEDCVAFGKGENAKKYLRTDDRVERVRKAHLNDLENIIPFLGIGLLYSLSGPDPST
AILHFRLFVGARIYHTIAYLTPLPQPNRALSFFVGYGVTLSMAYRLLKSKLYL"
BASE COUNT 131 a 102 c 93 g 139 t
ORIGIN
1 atggttgacc tcacccaggt aatggatgat gaagtattca tggcttttgc atcctatgca
61 acaattattc tttcaaaaat gatgcttatg agtactgcaa ctgcattcta tagattgaca
121 agaaaggttt ttgccaatcc agaagactgt gtagcatttg gcaaaggaga aaatgccaag
181 aagtatcttc gaacagatga cagagtagaa cgtgtacgca aagcccacct gaatgacctt
241 gaaaatatta ttccatttct tggaattggc ctcctgtatt ccttgagtgg tcccgacccc
301 tctacagcca tcctgcactt cagactattt gtcggagcac ggatctacca caccattgca
361 tatttgacac cccttcccca gccaaataga gctttgagtt tttttgttgg atatggagtt
421 actctttcca tggcttacag gttgctgaaa agtaaattgt acctg
//