LOCUS CR407628 435 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D042D for gene UBE2V2, ubiquitin-conjugating enzyme E2 variant 2 complete cds, without stopcodon. ACCESSION CR407628 VERSION CR407628.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 435) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 435) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D042D, ORFNo 403 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D042D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Ina Rolfs RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 101 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon (ATG): att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG) After the last codon additional sequence has been added: CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC TT). Compared to the reference sequence NM_003350 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..435 /db_xref="H-InvDB:HIT000267125" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D042D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>435 /codon_start=1 /gene="UBE2V2" /db_xref="GOA:Q15819" /db_xref="H-InvDB:HIT000267125.13" /db_xref="HGNC:HGNC:12495" /db_xref="InterPro:IPR000608" /db_xref="InterPro:IPR016135" /db_xref="PDB:1J74" /db_xref="PDB:1J7D" /db_xref="PDB:1ZGU" /db_xref="PDB:3VON" /db_xref="PDB:4NR3" /db_xref="PDB:4NRG" /db_xref="PDB:4NRI" /db_xref="PDB:4ONL" /db_xref="PDB:4ONM" /db_xref="PDB:4ONN" /db_xref="PDB:4ORH" /db_xref="PDB:5AIT" /db_xref="UniProtKB/Swiss-Prot:Q15819" /protein_id="CAG28556.1" /translation="MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLT RWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARS IPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN" BASE COUNT 160 a 75 c 101 g 99 t ORIGIN 1 atggcggtct ccacaggagt taaagttcct cgtaattttc gcttgttgga agaacttgaa 61 gaaggacaaa aaggagtagg cgacggtaca gttagctggg gccttgaaga tgatgaagat 121 atgacactta caaggtggac aggcatgatt attgggccac caaggacaaa ttatgaaaac 181 agaatatata gcctgaaagt agaatgtgga cctaaatacc cagaagctcc tccgtcagtt 241 agatttgtaa caaaaattaa tatgaacgga ataaataatt ccagtgggat ggtggatgcc 301 cggagcatac cagtgttagc aaaatggcaa aattcatata gcattaaagt tgtacttcaa 361 gagctaagac gtctaatgat gtccaaagaa aatatgaagc ttccacagcc accagaagga 421 caaacataca acaat //