LOCUS CR407622 387 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C052D for gene LAMA4, laminin, alpha 4 complete cds, without stopcodon. ACCESSION CR407622 VERSION CR407622.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 387) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 387) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834C052D, ORFNo 386 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C052D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Ina Rolfs RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 101 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon (ATG): att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG) After the last codon additional sequence has been added: CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC TT). Compared to the reference sequence U77706 we found amino acid exchange(s) at position (first base of changed triplet): 334(thr->ala) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..387 /db_xref="H-InvDB:HIT000267119" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834C052D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>387 /codon_start=1 /gene="LAMA4" /db_xref="H-InvDB:HIT000267119.11" /db_xref="HGNC:HGNC:6484" /db_xref="UniProtKB/TrEMBL:Q6LET9" /protein_id="CAG28550.1" /translation="MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGR QDPPETSEPRVALGRLPPAAEKCNAGFFHTLSGECVPCDCNGNSNECLDGSGYCVTTD GEDPGFSASALSFILSSQGAREARAPT" BASE COUNT 70 a 115 c 115 g 87 t ORIGIN 1 atggctttga gctcagcctg gcgctcggtt ctgcctctgt ggctcctctg gagcgctgcc 61 tgctcccgcg ccgcgtccgg ggacgacaac gcttttcctt ttgacattga agggagctca 121 gcggttggca ggcaagaccc gcctgagacg agcgaacccc gcgtggctct gggacgcctg 181 ccgcctgcgg ccgagaaatg caatgctgga ttctttcaca ccctgtcggg agaatgtgtg 241 ccctgcgact gtaatggcaa ttccaacgag tgtttggacg gctcaggata ctgtgtgact 301 actgacggag aagacccagg tttttcagct tctgccctat cgttcattct cagctctcag 361 ggagccagag aagccagggc tccaaca //