LOCUS       CR407622                 387 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834C052D for
            gene LAMA4, laminin, alpha 4 complete cds, without stopcodon.
ACCESSION   CR407622
VERSION     CR407622.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 387)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
            D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834C052D, ORFNo 386
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C052D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Ina Rolfs
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 101
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon
            (ATG):
            att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
            After the last codon additional sequence has been added:
            CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
            TT).
            Compared to the reference sequence U77706 we found amino acid
            exchange(s) at position (first base of changed triplet):
            334(thr->ala)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..387
                     /db_xref="H-InvDB:HIT000267119"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834C052D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>387
                     /codon_start=1
                     /gene="LAMA4"
                     /db_xref="H-InvDB:HIT000267119.11"
                     /db_xref="HGNC:HGNC:6484"
                     /db_xref="UniProtKB/TrEMBL:Q6LET9"
                     /protein_id="CAG28550.1"
                     /translation="MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGR
                     QDPPETSEPRVALGRLPPAAEKCNAGFFHTLSGECVPCDCNGNSNECLDGSGYCVTTD
                     GEDPGFSASALSFILSSQGAREARAPT"
BASE COUNT           70 a          115 c          115 g           87 t
ORIGIN      
        1 atggctttga gctcagcctg gcgctcggtt ctgcctctgt ggctcctctg gagcgctgcc
       61 tgctcccgcg ccgcgtccgg ggacgacaac gcttttcctt ttgacattga agggagctca
      121 gcggttggca ggcaagaccc gcctgagacg agcgaacccc gcgtggctct gggacgcctg
      181 ccgcctgcgg ccgagaaatg caatgctgga ttctttcaca ccctgtcggg agaatgtgtg
      241 ccctgcgact gtaatggcaa ttccaacgag tgtttggacg gctcaggata ctgtgtgact
      301 actgacggag aagacccagg tttttcagct tctgccctat cgttcattct cagctctcag
      361 ggagccagag aagccagggc tccaaca
//