LOCUS CR407618 243 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1116D for
gene NDUFA4, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4,
9kDa complete cds, without stopcodon.
ACCESSION CR407618
VERSION CR407618.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 243)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 243)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1116D, ORFNo 267
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1116D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_002489 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..243
/db_xref="H-InvDB:HIT000267115"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1116D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>243
/codon_start=1
/gene="NDUFA4"
/db_xref="GOA:O00483"
/db_xref="H-InvDB:HIT000267115.13"
/db_xref="HGNC:HGNC:7687"
/db_xref="InterPro:IPR010530"
/db_xref="PDB:5Z62"
/db_xref="UniProtKB/Swiss-Prot:O00483"
/protein_id="CAG28546.1"
/translation="MLRQIIGQAKKHPSLIPLFVFIGTGATGATLYLLRLALFNPDVC
WDRNNPEPWNKLGPNDQYKFYSVNVDYSKLKKERPDF"
BASE COUNT 67 a 58 c 56 g 62 t
ORIGIN
1 atgctccgcc agatcatcgg tcaggccaag aagcatccga gcttgatccc cctctttgta
61 tttattggaa ctggagctac tggagcaaca ctgtatctct tgcgtctggc attgttcaat
121 ccagatgttt gttgggacag aaataaccca gagccctgga acaaactggg tcccaatgat
181 caatacaagt tctactcagt gaatgtggat tacagcaagc tgaagaagga acgtccagat
241 ttc
//