LOCUS CR407614 615 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B022D for
gene HSPB1, heat shock 27kDa protein 1 complete cds, without
stopcodon.
ACCESSION CR407614
VERSION CR407614.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 615)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 615)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (07-MAY-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH Im Neuenheimer Feld 580,
D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B022D, ORFNo 256
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B022D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Ina Rolfs
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 101
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon
(ATG):
att..AAAAAA GCT GGC ACC CCT GGT CCA GGT (ATG)
After the last codon additional sequence has been added:
CCA GGC CCA GGC GGC G in front of the 3' att site (AC CCA GCT TTC
TT).
Compared to the reference sequence NM_001540 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..615
/db_xref="H-InvDB:HIT000267111"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B022D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>615
/codon_start=1
/gene="HSPB1"
/db_xref="GOA:P04792"
/db_xref="H-InvDB:HIT000267111.13"
/db_xref="HGNC:HGNC:5246"
/db_xref="InterPro:IPR001436"
/db_xref="InterPro:IPR002068"
/db_xref="InterPro:IPR008978"
/db_xref="InterPro:IPR037876"
/db_xref="PDB:2N3J"
/db_xref="PDB:3Q9P"
/db_xref="PDB:3Q9Q"
/db_xref="PDB:4MJH"
/db_xref="PDB:6DV5"
/db_xref="PDB:6GJH"
/db_xref="UniProtKB/Swiss-Prot:P04792"
/protein_id="CAG28542.1"
/translation="MTERRVPFSLLRGPSWDPFRDWYPHSRLFDQAFGLPRLPEEWSQ
WLGGSSWPGYVRPLPPAAIESPAVAAPAYSRALSRQLSSGVSEIRHTADRWRVSLDVN
HFAPDELTVKTKDGVVEITGKHEERQDEHGYISRCFTRKYTLPPGVDPTQVSSSLSPE
GTLTVEAPMPKLATQSNEITIPVTFESRAQLGGPEAAKSDETAAK"
BASE COUNT 105 a 234 c 188 g 88 t
ORIGIN
1 atgaccgagc gccgcgtccc cttctcgctc ctgcggggcc ccagctggga ccccttccgc
61 gactggtacc cgcatagccg cctcttcgac caggccttcg ggctgccccg gctgccggag
121 gagtggtcgc agtggttagg cggcagcagc tggccaggct acgtgcgccc cctgcccccc
181 gccgccatcg agagccccgc agtggccgcg cccgcctaca gccgcgcgct cagccggcaa
241 ctcagcagcg gggtctcgga gatccggcac actgcggacc gctggcgcgt gtccctggat
301 gtcaaccact tcgccccgga cgagctgacg gtcaagacca aggatggcgt ggtggagatc
361 accggcaagc acgaggagcg gcaggacgag catggctaca tctcccggtg cttcacgcgg
421 aaatacacgc tgccccccgg tgtggacccc acccaagttt cctcctccct gtcccctgag
481 ggcacactga ccgtggaggc ccccatgccc aagctagcca cgcagtccaa cgagatcacc
541 atcccagtca ccttcgagtc gcgggcccag cttgggggcc cagaagctgc aaaatccgat
601 gagactgccg ccaag
//