LOCUS CK335189 699 bp mRNA linear EST 12-MAY-2010
DEFINITION H3150G10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3150G10 5', mRNA sequence.
ACCESSION CK335189
VERSION CK335189.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 699)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3150 row: G column: 10
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..699
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3150G10-5"
/db_xref="taxon:10090"
/clone="H3150G10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 221 a 146 c 134 g 198 t
ORIGIN
1 ctcacattgt agtctcagca tttgggaggc tgaggcagaa agattaccag gagttggaga
61 caaatcctag gttacagtgt gagatactat cccccaaaac atccccacaa acaaaaacac
121 tagtgtattg ttttgccgta aagaatatct ctggggccta ggaaaacgtt cagtcagtaa
181 tggactcaca ccagccttag gacctgagtt taaacttgga gggagcagat ccctggattt
241 tacgaggtag ccaaactcag cctaactagt gagccccaga tccaaggggg aaatcctgta
301 ttccaaaaag caaggtggat gcctcctgag gaagggccgc ctagactgat ccctagcctc
361 cacatccaca cacacacatg cacacattca gaaatatgga tgtgtacaca caaaaaggat
421 atctcaggat gctgttactc tgccattaaa agtttataat catgaatttt taaaatactt
481 gattatgcac attatggaat acgtttctgt ttccagataa ctaacttgta accaatcatt
541 ttatttccta atatcctttt catattaaga gaattatatg tgactaaaaa tatacatgat
601 ttgtgtttgt taagcatttt aaatttgtct ttgcttttga caataagaac caaaatttct
661 tccgtgcgcc tttgtcagct aagaatgact agcagtggc
//