LOCUS CK335174 667 bp mRNA linear EST 12-MAY-2010 DEFINITION H3150B06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3150B06 5', mRNA sequence. ACCESSION CK335174 VERSION CK335174.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 667) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3150 row: B column: 06 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..667 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3150B06-5" /db_xref="taxon:10090" /clone="H3150B06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 142 a 149 c 189 g 187 t ORIGIN 1 ctttttgtta gtctgctttg aatttcactg catagcccgt gttggcttct aagtctggaa 61 aattctgctt accgatgttg taaatgacag cgttgctagg ggacttggtg agttacgtag 121 tttctttatt caataaagct taatgtccca agtcttctaa catctggcca atgtgggaca 181 ggtgagcaga tgaagcactg tggatactgt ttgtgcctag tataggctta tgtccccaac 241 gctgtaggac cccagctatg cacctcagag ccctagaggg ggccccgtgg agacttgtta 301 ctccggtaaa cagctgtgag gttgggagtg ggctttgcag ctctgcttcc agactctggg 361 tgctcagctc tttccatcag ttctctctca cctggcagag aggttcgaga ttgctggggt 421 tgaggggaca gccactttcc tggccatggt ggagcagctt accattttgt acctatttgt 481 ccggtggcag gaaaggaaag ctgccatttg gggatagtca cggagccctg aaggcacaga 541 acagagtgtt ggctacagtt gacagagagg gtctgctatt ctatgggtgg cacgggctca 601 gccatattac ctagcattac ggacagggtc ccctactcct tactgcagag gatgggtgtg 661 aatctga //