LOCUS CK335156 482 bp mRNA linear EST 12-MAY-2010 DEFINITION H3148F09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3148F09 5', mRNA sequence. ACCESSION CK335156 VERSION CK335156.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 482) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3148 row: F column: 09 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..482 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3148F09-5" /db_xref="taxon:10090" /clone="H3148F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 120 a 127 c 161 g 74 t ORIGIN 1 ctgggcccag aacccaggat ggacccagaa tctgagggag cctcccaggc ccttcgggac 61 tcctccaagg cagatggcaa agaattagct gggaccttgg atggagaaga gaagcttttc 121 cagacggaaa gttcccagag agaaactgct gttctgacag agtctgcggc caagggcact 181 ctgggagccg atggtcatgg gacggaggcc cctggagaca cagtggtcca ggaagattcg 241 caggagactc ctgtggcgac gagcctcgga ccagacacac aggacctgga gagtgagatc 301 catccacaga acttaccctc cagtcccaga gcagtttgga aagagcatcg ctgctccagc 361 agtgacgacg acactgatgt agatgtggag ggtctgcgga gacggagggg ccgggagccc 421 agctcatccc agcccgtggt acccgtagat gtggaggacc aggccaaggg cgagggtata 481 gg //