LOCUS       CK335141                 620 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3147D09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3147D09 5', mRNA sequence.
ACCESSION   CK335141
VERSION     CK335141.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     Contact: Dawood B. Dudekula
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            Plate: H3147  row: D  column: 09
            Seq primer: M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3147D09-5"
                     /db_xref="taxon:10090"
                     /clone="H3147D09"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          160 a          184 c          188 g           88 t
ORIGIN      
        1 agcaacgaca agccggataa ccgctcttga gacaggatga cgtggcatgg caagaactgc
       61 actgcagggg ccgtctacac ctaccacgag aagaagaagg acacagcggc ctcgggctat
      121 gggacccaga acattctact gagccgggac gctgtgaaag actttgactg ctgctgtctc
      181 tctctgcagc cctgccatga ccctgtggtc acgccagacg gctacctgta tgagagggaa
      241 gcgatcctgg agtacatcct gcaccagaag agagagatcg cccggcaggt gaaggcctat
      301 gagaagcagc gaggagcgag gagagaagag cagaaagagc tgcagcgagc tgcagcccag
      361 gaccaagtta gaggcttcct agagaaggag gcggccatcg tgagccggcc cctcaacccc
      421 ttcatgccca aggctgccac cttgcccaac acagagggtg aacagccagg gcccagtgtg
      481 ggtcccgtag gcaaggacaa ggacaaagca ctgcccagct tctggattcc ctcactaacg
      541 cccgaggcaa aggccaccaa gctggaaaag ccgtcccgca cggtgacctg cccgatgtcc
      601 gggaagccac tgcgcatgtc
//