LOCUS CK335086 571 bp mRNA linear EST 12-MAY-2010 DEFINITION H3140B05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3140B05 5', mRNA sequence. ACCESSION CK335086 VERSION CK335086.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 571) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3140 row: B column: 05 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..571 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3140B05-5" /db_xref="taxon:10090" /clone="H3140B05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 168 c 175 g 107 t ORIGIN 1 gcccaggaga ggcgctccgc ccgcggcgta aactctaccc cgcgccgcgg cggagcagac 61 catgctcgca ttcatggcct ctgacagcga ggaagaggtg tgtgatgagc ggacgtcctt 121 gatgtcagcc gagagcccca catctcgctc ctgccaggaa ggcaggccag gcccggagga 181 tggagagagc actgcccagt ggaggactca ggagagcgaa gaagactgtg aagaggaccc 241 ggaccgctac gcatgcagtg gggctcctgg gcgaccgtcg ggcctggagg aagagctgac 301 cctcaagtat ggggcgaagc atgtgatcat gctattcgtg cctgtcacgc tgtgtatgat 361 cgtggtggtg gccactatca agtctgtgcg tttctacact gagaagaacg ggcagctcat 421 ctacacgccc ttcacggagg acacgccctc ggtgggccag cggctcctca actccgtgct 481 taacaccctc atcatgatca gcgtcatcgt agtcatgacc atcttcctcg tggtactcta 541 caagtatcga tgctacaagt tcatccatgg c //