LOCUS CK334883 526 bp mRNA linear EST 12-MAY-2010 DEFINITION H3118C07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3118C07 5', mRNA sequence. ACCESSION CK334883 VERSION CK334883.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 526) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3118 row: C column: 07 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..526 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3118C07-5" /db_xref="taxon:10090" /clone="H3118C07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 143 a 122 c 142 g 119 t ORIGIN 1 ccgcagacgg cctgcctcgc ctcgtgctct cctgccgccg ccccgcagaa atgcttcgac 61 tacccacagt ccttcgccag atgagaccag tgtcccgggc actggctcct catctcactc 121 gggcctatgc caaagatgta aaatttggtg cggacgctcg agccttaatg cttcaaggtg 181 tagacctttt agcagatgct gtagctgtta caatggggcc aaagggaaga actgtgatta 241 ttgaacagag ttggggaagt cccaaagtaa caaaagatgg ggtcactgtt gcaaagtcaa 301 ttgatttaaa ggataaatac aaaaatattg gagctaaact tgttcaggac gttgccaata 361 acacaaacga agaggctggg gatggcacca ccactgccac tgttctggca cgatctattg 421 ccaagggggg ctttgagaag atcagcaaag gggctaatcc agtggaaatc cggagaggtg 481 tgatgttggc tgtggatgct gtaattgctg aacttaagaa acagtc //