LOCUS CK334866 578 bp mRNA linear EST 12-MAY-2010 DEFINITION H3117H03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3117H03 5', mRNA sequence. ACCESSION CK334866 VERSION CK334866.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 578) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3117 row: H column: 03 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..578 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3117H03-5" /db_xref="taxon:10090" /clone="H3117H03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 138 a 157 c 133 g 150 t ORIGIN 1 attgttacag tggtcagggt tcagatagcc cagttttcta ggctttcaaa catcatgtgt 61 ccttgtccca tccacagtct cgatgtgctt gggagagtct cctggctgtg ctcacagacc 121 ctgccctggc tcctggggac cactaagatc acatcaactc tttctctccc attaccatcc 181 tcctggtcca acctgttctc cctggagatc tccgaattgc tggcctctct ccatcccttt 241 tgctacctcc tgcatacagc cctgaagcta ccttggcagg gtcctccacg gagtaacagt 301 gggacccaga aaatgaaggt gggaagtcgg gaatggtgac acatatttat aattccactg 361 aggccttgag gttagcctgg gctacagggt gagctcttat ctcaaaaaac aatgacatta 421 aaagtataag tgggaggctg gagagatggc tcagtggtta agagcactga ctgctcttct 481 gaaggtcctg agttcaaatc ccagcaacca catggtagct cacaaccatc cataatgaga 541 tctgatgccc tcttctggtg cacctgaaga cagctaca //