LOCUS CK334847 497 bp mRNA linear EST 12-MAY-2010 DEFINITION H3117B01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3117B01 5', mRNA sequence. ACCESSION CK334847 VERSION CK334847.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 497) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3117 row: B column: 01 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..497 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3117B01-5" /db_xref="taxon:10090" /clone="H3117B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 132 a 112 c 133 g 120 t ORIGIN 1 gcctgttcaa agtcctgcat aaggggatcc ctgtacactc gaaccgttat tatttgatga 61 tgtaaaagtc atagtgagat gcttacagga gaacccgcag actagctaga gagtgtgcgt 121 acctgcaaca tgggaacgaa ttagaggaga ctgtcttttg tgcttgtgac ctagtgcata 181 tgcccccttt agtagaattg caaggaaccg tgggtgccag gaccgcatga gtagtttgca 241 aagcagtgaa cccaaggagg aatcagaaac gagaagggac atgccgggga ggttgcgcaa 301 aaccacccaa ccacatgact gaaccatttt ggatgtaaga agcgccttga tgcctcacca 361 cgtgttccca agactgagcg tccgggtctc tccgtcgcat actcttgctg actaaagtag 421 ctgctaccta agggatatgt cttttgccag ttggacacag gtgaacggct cctaagtctc 481 atgtttggtg aatttgg //