LOCUS CK334768 647 bp mRNA linear EST 12-MAY-2010
DEFINITION H3107C09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3107C09 5', mRNA sequence.
ACCESSION CK334768
VERSION CK334768.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 647)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3107 row: C column: 09
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..647
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3107C09-5"
/db_xref="taxon:10090"
/clone="H3107C09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 209 a 141 c 189 g 108 t
ORIGIN
1 cacatccaca tgaacgctac ccagtgggag acactgaccg actttaccaa gtggctgggc
61 agagaggtgc tgtcggactc ggagtccatg gatggattaa ttcactgatg gagtcagggt
121 ctccagatcc agtcacttca caatgcctgg agaccaagct ctcagcacat gagctctggg
181 aaacggcttg tgtaaagtgg atgagacacc gaaaggctgg tacattcagt acatagacag
241 agacccagaa accatccgtc ggcaactgga attagaaaaa aagaagaagc aagatctgga
301 cgatgaagaa aaaactgcca agttcattga ggagcaggtg agaagaggcc tggaagggaa
361 agagcaggag acacctgttt ttacagaact tagccgagaa aatgaggaag aaaaagttac
421 gttcaatctg aataaaggag cgggtggctc agcgggagct acaacatcca agtcaagctc
481 tttgggacca agtgcactga agctgctggg gagcgcagca tccgggaaac ggaaagagtc
541 ttcacagagc tccgcccagc ctgcgaagaa gaagaagtcg gccctggatg agatcatgga
601 gctcgaagag gaaaagaaaa ggaccgcacg gacagacgcc tggttac
//