LOCUS CK334710 631 bp mRNA linear EST 12-MAY-2010 DEFINITION H3098B08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3098B08 5', mRNA sequence. ACCESSION CK334710 VERSION CK334710.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 631) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3098 row: B column: 08 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..631 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3098B08-5" /db_xref="taxon:10090" /clone="H3098B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 133 c 142 g 193 t ORIGIN 1 ctctatgcca gctctgggac ttatttgttg accatcctgg cctcaaattt gcagtgatgc 61 tccagcctca gcctcctcaa tggtggggtt atagggttat attgcctagc aatagtgggt 121 gctatttatc ttttgtttct ttagacagag tctcttgtag accaggctag ccttagatta 181 ctgtatagcc caggatggcc ttgaatttct gataatcttg cctctacctt ctaagtgctg 241 ggattacagg tttgtggcac cacagccagc ataacggatg ctttttaaag gctttattga 301 ctaggcatgg tggtacatgc ccatcaatca cagcactcgc agggcggctt agactatatt 361 aaggagctcc tgcctcaaaa aacaagacag aaaacttttt aaaactgaaa agttgtatta 421 ttgttatgtg ctccaattgt gttcttaaaa taaactgtca cacttcaggt ttgtttttct 481 aagatcctgg attctatgga gtgtcctaga cccgttaata gcaggattct gcaaggctga 541 gtctcagatt cttaggttat acaactggac tggaatgtca ccaggcagga tggccagagc 601 tatgagatgg agagacccag tctcaaaaag c //