LOCUS CK334708 658 bp mRNA linear EST 12-MAY-2010
DEFINITION H3098B03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3098B03 5', mRNA sequence.
ACCESSION CK334708
VERSION CK334708.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 658)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT Contact: Dawood B. Dudekula
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
Plate: H3098 row: B column: 03
Seq primer: M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..658
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3098B03-5"
/db_xref="taxon:10090"
/clone="H3098B03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 184 a 169 c 150 g 155 t
ORIGIN
1 attcttattt caacctagca ggccagggct ccccaaatat atatgaccat gaagtcacca
61 ttgcagcaga tgcatatttg cccgtggatg aaactctgat tccaacagga gtcattgctc
121 cagtggaagg aactgccttc gacctgagaa agccagtgga gcttggaaca cacctgcagg
181 attaccacat ccatggtttc gaccataatt tctgtctgaa ggagtcaaaa gaaaagaagt
241 tttgtgcaag ggtgcgtcac gctgcaagtg ggaggatcct ggaagtatat accacacaac
301 caggcgtcca gttttacaca ggaaacttct tggatggtac tctgaaaggc aagaacggtg
361 cggtgtatcc caagcactca ggtctctgcc tcgagaccca gaactggcct gattcggtca
421 atcagcccca gttccctccg gcgctgctga ggcctggaga ggaatacaac cacactacct
481 ggttcaagtt ctctgttgct taaagaagac ggaagatgag acccggtccc cactcacctg
541 ccttgcttta aaagtaatcc tgcgtgttaa atcatacaga tcgtagcagc cataataatc
601 aatgtgaata ctgataccct ttcccagcag ctacagacta tgtctaatga ccagctct
//