LOCUS CK334677 511 bp mRNA linear EST 12-MAY-2010 DEFINITION H3085F08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3085F08 5', mRNA sequence. ACCESSION CK334677 VERSION CK334677.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 511) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT Contact: Dawood B. Dudekula Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov Plate: H3085 row: F column: 08 Seq primer: M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..511 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3085F08-5" /db_xref="taxon:10090" /clone="H3085F08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 126 a 97 c 120 g 168 t ORIGIN 1 cagaaatgaa atggtttgcc aggctgtggt agatgaaggc tgagctctgt gagtttgtgg 61 tcagcctggg ctacatagtg agaccctgta tccaggggaa aagaaaaaaa aaggaaggaa 121 ggaagaaaag tagcgtgttt tcaagctgag gctggggctc agaggtatag tgcttgccta 181 gcaagagtta cactctgtgt tcaatcccaa tgccttagaa agatttcttg aaacctggtc 241 tgtgaatttt tccttgtttt gataaggact gttccttgaa acctggtctt ttgtttttta 301 aaacacatgt tcgtaaaaca tggggctatt tttttaaaat aagtgtttct tgaaacctat 361 tatggctctt gttttcattt ttgtttttgt gtgccggttt tctaaaagca atgttcttga 421 aacctgtctt tgagacagag ctctcgctgc tcttacctct cttagggaga gctgccccgc 481 cttcagctca cattcatcct cctgtctcag c //